{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:30:04Z","timestamp":1781656204172,"version":"3.54.5"},"reference-count":40,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2002,12,12]],"date-time":"2002-12-12T00:00:00Z","timestamp":1039651200000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>We previously reported that B cells lacking the RP105 molecule, which proved to be highly activated B cells, are increased in the peripheral blood of patients with systemic lupus erythematosus (SLE). In the present study, we attempted to determine whether RP105\u2010negative B cells obtained from SLE patients would be capable of producing autoantibodies as well as immunoglobulins.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>RP105\u2010positive and RP105\u2010negative B cells, sorted by cell sorter, were cultured for 5 days without stimulation, or were stimulated with <jats:italic>Staphylococcus aureus<\/jats:italic> Cowan 1 strain (SAC) or recombinant interleukin\u20106 (IL\u20106). For the assay of autoantibodies, RP105\u2010positive and RP105\u2010negative B cells were cultured separately for 10 days with anti\u2010CD3 antibody\u2013stimulated T cells. The production of immunoglobulins and autoantibodies was determined by enzyme\u2010linked immunosorbent assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We demonstrated that RP105\u2010negative B cells, but not RP105\u2010positive B cells, obtained from SLE patients could spontaneously produce IgG and IgM in vitro until day 5. SAC and IL\u20106 enhanced production of IgG and IgM by RP105\u2010negative B cells but failed to induce such production by RP105\u2010positive B cells. The latter cells, however, when cocultured with activated T cells in the presence of IL\u201010, produced IgG, although the amount was very small compared with that produced by RP105\u2010negative B cells. Most important, under these conditions, anti\u2013double\u2010stranded DNA antibodies were produced only by the RP105\u2010negative B cells obtained from SLE patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These data indicate that RP105\u2010negative B cells, constituting a subset of B cells in SLE patients, are highly activated and may be responsible for the production of autoantibodies as well as polyclonal immunoglobulins.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.10672","type":"journal-article","created":{"date-parts":[[2002,12,16]],"date-time":"2002-12-16T13:50:56Z","timestamp":1040046656000},"page":"3259-3265","source":"Crossref","is-referenced-by-count":34,"title":["RP105\u2010lacking B cells from lupus patients are responsible for the production of immunoglobulins and 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