{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:02:00Z","timestamp":1791219720056,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,2,4]],"date-time":"2003-02-04T00:00:00Z","timestamp":1044316800000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2003,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate the repair of partial\u2010thickness lesions in rat articular cartilage by combining cell transplantation with transfer of growth factor complementary DNA (cDNA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mesenchymal cells isolated from rib perichondrium were infected ex vivo with adenoviral vectors carrying bone morphogenetic protein 2 (BMP\u20102) or insulin\u2010like growth factor 1 (IGF\u20101) cDNA. The cells were suspended in fibrin glue and applied to mechanically induced partial\u2010thickness cartilage lesions in the patellar groove of the rat femur. The filling of the defects was quantified and the quality and integration of the newly formed tissue were assessed by histochemical and immunohistochemical methods. Uninfected cells or cells infected with a LacZ reporter gene vector served as controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Transplanted cells were able to attach to the wounded articular cartilage and were not displaced from the lesions by joint movement. Cells infected with both adenoviral vectors AdBMP\u20102 and AdIGF\u20101 produced repair cartilage of hyaline morphology containing a type II collagen\u2013positive but type I collagen\u2013negative proteoglycan\u2010rich matrix that restored the articular surface in most lesions. Uninfected cells either failed to fill up the defects or formed fibrous tissue mainly composed of type I collagen. Excessive cells were partially dislocated to the joint margins, leading to osteophyte formation there if AdBMP\u20102\u2013infected cells were used. These adverse effects, however, were not seen with AdIGF\u20101\u2013infected cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Stimulation of perichondrium\u2010derived mesenchymal cells by transfer of growth factor cDNA in a partial\u2010thickness defect model allows for satisfactory cartilage restoration by a repair tissue comparable with hyaline articular cartilage.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.10759","type":"journal-article","created":{"date-parts":[[2003,2,7]],"date-time":"2003-02-07T07:09:02Z","timestamp":1044601742000},"page":"430-441","source":"Crossref","is-referenced-by-count":202,"title":["Articular cartilage repair by gene therapy using growth factor\u2013producing mesenchymal cells"],"prefix":"10.1002","volume":"48","author":[{"given":"Kolja","family":"Gelse","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"von der 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