{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T22:25:17Z","timestamp":1792448717392,"version":"4.5.0"},"reference-count":24,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2003,7,2]],"date-time":"2003-07-02T00:00:00Z","timestamp":1057104000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>T cells from a majority of patients with systemic lupus erythematosus (SLE) display antigen receptor\u2013mediated signaling aberrations associated with defective T cell receptor (TCR) \u03b6 chain, a subunit of the TCR\/CD3 complex. This study was undertaken to explore the possibility that forced expression of TCR \u03b6 chain may reverse the known signaling abnormalities and defective interleukin\u20102 (IL\u20102) production in SLE T cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Freshly isolated SLE T cells were transfected with TCR \u03b6 chain construct in a eukaryotic expression vector at high efficiency, by a recently developed nucleoporation technique. Restoration of TCR\/CD3\u2010mediated signaling was studied in the \u03b6 chain\u2013transfected cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In SLE T cells transfected with TCR \u03b6 chain, surface expression of TCR chain was increased and the TCR\/CD3\u2010induced increased free intracytoplasmic calcium concentration response was normalized, as was hyperphosphorylation of cellular substrates. Simultaneously, the previously noted increased expression of the Fc receptor \u03b3 chain was diminished in SLE T cells transfected with the \u03b6 chain expression vector, and the surface membrane clusters of cell signaling molecules were redistributed to a more continuous pattern. TCR \u03b6 chain replacement also augmented the expression of diminished TCR\/CD3\u2010mediated IL\u20102 production in SLE T cells, associated with increased expression of the p65 subunit of nuclear factor \u03baB in the nuclear fractions of these T cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results suggest that reconstitution of deficient TCR \u03b6 chain can reverse the TCR\/CD3\u2010mediated signaling abnormalities as well as the defective IL\u20102 production in T cells of patients with SLE.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.11072","type":"journal-article","created":{"date-parts":[[2003,7,3]],"date-time":"2003-07-03T10:03:27Z","timestamp":1057226607000},"page":"1948-1955","source":"Crossref","is-referenced-by-count":92,"title":["Reconstitution of deficient T cell receptor \u03b6 chain restores T cell signaling and augments T cell receptor\/CD3\u2013induced interleukin\u20102 production in patients with systemic lupus erythematosus"],"prefix":"10.1002","volume":"48","author":[{"given":"Madhusoodana P.","family":"Nambiar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn U.","family":"Fisher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vishal G.","family":"Warke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandeep","family":"Krishnan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeanne P.","family":"Mitchell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy","family":"Delaney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George C.","family":"Tsokos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,7,2]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.10192"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI1457"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/jaut.1998.0223"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(199909)42:9<1908::AID-ANR17>3.0.CO;2-7"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1191\/0961203303lu281oa"},{"key":"e_1_2_5_7_2","doi-asserted-by":"crossref","first-page":"2269","DOI":"10.4049\/jimmunol.155.4.2269","article-title":"TCR\/CD3 complex\u2010mediated signal transduction pathway in T cells and cell lines from patients with systemic lupus erythematosus","volume":"155","author":"Vassilopoulos D","year":"1995","journal-title":"J 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