{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T09:07:17Z","timestamp":1791104837189,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2003,7,2]],"date-time":"2003-07-02T00:00:00Z","timestamp":1057104000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To identify and recombine a protein of the human dermal microvascular endothelial cell (HDMEC) that specifically reacts with anti\u2013endothelial cell antibody (AECA) in the serum of patients with Beh\u00e7et's disease (BD), and to evaluate the usefulness of this protein in BD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The proteomics technique, with 2\u2010dimensional gel electrophoresis and matrix\u2010assisted laser desorption ionization\u2212time\u2010of\u2010flight (MALDI\u2010TOF) mass spectrometry, was used to identify and recombine HDMEC antigen. Western blotting and enzyme\u2010linked immunosorbent assay (ELISA) of recombinant protein isolated by gene cloning were performed on serum from healthy controls, patients with BD, and patients with other rheumatic diseases (rheumatoid arthritis, systemic lupus erythematosus, and Wegener's granulomatosis).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Eighteen of 40 BD patients had serum IgM antibody to HDMEC antigen. The purified protein that reacted with AECA in BD patient sera was found to be \u03b1\u2010enolase by 2\u2010dimensional gel electrophoresis followed by immunoblotting and MALDI\u2010TOF mass spectrometry. Recombinant \u03b1\u2010enolase protein was isolated and refined by gene cloning. On Western blots, AECA\u2010positive IgM from the sera of patients with active BD reacted strongly with recombinant human \u03b1\u2010enolase. BD patient sera positive for anti\u2013\u03b1\u2010enolase did not react with human \u03b3\u2010enolase. On dot\u2010blotting, reactivity to human \u03b1\u2010enolase was detected only in the IgM\u2010positive group. Fifteen of the 18 AECA\u2010positive sera that were positive for the HDMEC antigen showed reactivity to recombinant \u03b1\u2010enolase IgM antibody by ELISA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The \u03b1\u2010enolase protein is the target protein of serum AECA in BD patients. This is the first report of the presence of IgM antibodies to \u03b1\u2010enolase in endothelial cells from the serum of BD patients. Although further studies relating this protein to the pathogenesis of BD will be necessary, \u03b1\u2010enolase and its antibody may prove useful in the development of new diagnostic and treatment modalities in BD.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.11074","type":"journal-article","created":{"date-parts":[[2003,7,3]],"date-time":"2003-07-03T10:03:27Z","timestamp":1057226607000},"page":"2025-2035","source":"Crossref","is-referenced-by-count":162,"title":["Human \u03b1\u2010enolase from endothelial cells as a target antigen of anti\u2013endothelial cell antibody in Beh\u00e7et's disease"],"prefix":"10.1002","volume":"48","author":[{"given":"Kwang Hoon","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hae\u2010Shin","family":"Chung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyoung 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