{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:29:28Z","timestamp":1791404968455,"version":"4.3.3"},"reference-count":60,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2003,8,1]],"date-time":"2003-08-01T00:00:00Z","timestamp":1059696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"K. H. Hunter Charitable Foundation"},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Health and Medical Research Council (Australia)"},{"name":"Arthritis Society of Canada"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2003,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To examine the role of chemokines, S100A8, and S100A9 in neutrophil accumulation induced by the causative agent of gout, monosodium urate monohydrate (MSU) crystals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>MSU crystal\u2013induced neutrophil migration was studied in the murine air\u2010pouch model. Release of chemokines, S100A8, S100A9, and S100A8\/A9 in response to MSU crystals was quantified by enzyme\u2010linked immunosorbent assays. Recruited cells were counted following acetic blue staining, and the subpopulations were characterized by Wright\u2010Giemsa staining of cytospins.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MSU crystals induced the accumulation of neutrophils following injection in the air pouch, which correlated with the release of the chemokines CXCL1, CXCL2, CCL2, and CCL3. However, none of these was found to play an important role in neutrophil migration induced by MSU crystals by passive immunization with antibodies directed against each chemokine. S100A8, S100A9, and S100A8\/A9 were also found at high levels in the pouch exudates following injection of MSU crystals. In addition, injection of S100A8, S100A9, or S100A8\/A9 led to the accumulation of neutrophils in the murine air pouch, demonstrating their proinflammatory activities in vivo. Passive immunization with anti\u2010S100A8 and anti\u2010S100A9 led to a total inhibition of the accumulation of neutrophils. Finally, S100A8\/A9 was found at high concentrations in the synovial fluid of patients with gout.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>S100A8 and S100A8\/A9 are essential to neutrophil migration induced by MSU crystals. These results suggest that they might be involved in the pathogenesis of gout.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.11079","type":"journal-article","created":{"date-parts":[[2003,8,4]],"date-time":"2003-08-04T17:38:48Z","timestamp":1060018728000},"page":"2310-2320","source":"Crossref","is-referenced-by-count":161,"title":["Role of S100A8 and S100A9 in neutrophil recruitment in response to monosodium urate monohydrate crystals in the air\u2010pouch model of acute gouty arthritis"],"prefix":"10.1002","volume":"48","author":[{"given":"Carle","family":"Ryckman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaun R.","family":"McColl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Vandal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rinaldo","family":"de M\u00e9dicis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9","family":"Lussier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice E.","family":"Poubelle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe A.","family":"Tessier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,8]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002281-199507000-00017"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002281-199305040-00017"},{"key":"e_1_2_6_4_2","first-page":"1039","volume-title":"Inflammation: basic principles and clinical correlates","author":"McColl SR","year":"1999"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/anr.1780321114"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780340716"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.182.6.2019"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780250210"},{"key":"e_1_2_6_9_2","doi-asserted-by":"crossref","first-page":"5485","DOI":"10.4049\/jimmunol.152.11.5485","article-title":"Crystal\u2010induced neutrophil activation. 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