{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T19:53:22Z","timestamp":1792612402659,"version":"4.5.0"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2003,7,2]],"date-time":"2003-07-02T00:00:00Z","timestamp":1057104000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To determine the gene\u2010expression profile in dermal fibroblasts from type 1 tight\u2010skin (Tsk1) mice, and to examine the expression and potential fibrotic activity of monocyte chemoattractant protein 3 (MCP\u20103) in Tsk1 mouse and human systemic sclerosis (SSc) skin.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Complementary DNA microarrays (Atlas 1.2) were used to compare Tsk1 fibroblasts with non\u2010Tsk1 littermate cells at 10 days, 6 weeks, and 12 weeks of age. Expression of MCP\u20103 protein was assessed by Western blotting of fibroblast culture supernatants, and localized in the mouse and human skin biopsy samples by immunohistochemistry. Activation of collagen reporter genes by MCP\u20103 was explored in transgenic mouse fibroblasts and by transient transfection assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>MCP\u20103 was highly overexpressed by neonatal Tsk1 fibroblasts and by fibroblasts cultured from the lesional skin of patients with early\u2010stage diffuse cutaneous SSc. Immunolocalization confirmed increased expression of MCP\u20103 in the dermis of 4 of 5 Tsk1 skin samples and 14 of 28 lesional SSc skin samples, compared with that in matched healthy mice (n = 5) and human controls (n = 11). Pro\u03b12(I) collagen promoter\u2013reporter gene constructs were activated by MCP\u20103 in transgenic mice and by transient transfection assays. This response was maximal between 16 and 24 hours of culture and mediated via sequences within the proximal promoter. The effects of MCP\u20103 could be diminished by a neutralizing antibody to transforming growth factor \u03b2.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>We demonstrate, for the first time, overexpression of MCP\u20103 in early\u2010stage SSc and in Tsk1 skin, and suggest a novel role for this protein as a fibrotic mediator activating extracellular matrix gene expression in addition to promoting leukocyte trafficking. This chemokine may be an important early member of the cytokine cascade driving the pathogenesis of SSc.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/art.11164","type":"journal-article","created":{"date-parts":[[2003,7,3]],"date-time":"2003-07-03T06:03:27Z","timestamp":1057212207000},"page":"1979-1991","source":"Crossref","is-referenced-by-count":71,"title":["Monocyte chemoattractant protein 3 as a mediator of fibrosis: Overexpression in systemic sclerosis and the type 1 tight\u2010skin mouse"],"prefix":"10.1002","volume":"48","author":[{"given":"Voon H.","family":"Ong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lowri A.","family":"Evans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Shiwen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan B.","family":"Fisher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vineeth","family":"Rajkumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Abraham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol M.","family":"Black","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher P.","family":"Denton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,7,2]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002281-200111000-00010"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)07524-1"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1359-6101(01)00014-4"},{"key":"e_1_2_6_5_2","first-page":"517","article-title":"JE\/MCP\u20101: an early\u2010response gene encodes a monocyte\u2010specific cytokine","volume":"3","author":"Rollins BJ","year":"1991","journal-title":"Cancer Cells"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.176.1.59"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.5.2379"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.185.1.99"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.162.2.9907014"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115950"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9150(98)00285-8"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(98)00002-2"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.44.5.629"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1998.00721.x"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200106)44:6<1382::AID-ART231>3.0.CO;2-T"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200111)44:11<2665::AID-ART446>3.0.CO;2-S"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(2001010)31:10<2936::AID-IMMU2936>3.0.CO;2-0"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199802123380706"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.1999.00543.x"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.30.17779"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780230510"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/40.1.84"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200103)44:3<712::AID-ANR121>3.0.CO;2-1"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M101397200"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI1918"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(199907)42:7<1451::AID-ANR19>3.0.CO;2-6"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.4.1778"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.26.5.4683"},{"key":"e_1_2_6_29_2","first-page":"493","article-title":"Tight\u2010skin: a new mutation of the mouse causing excessive growth of connective tissue and skeleton","volume":"82","author":"Green MC","year":"1976","journal-title":"Am J Pathol"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780270209"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/4.4.267"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V100.4.1160.h81602001160_1160_1167"},{"issue":"9","key":"e_1_2_6_33_2","first-page":"54","article-title":"Pulmonary and activation\u2010regulated chemokine (PARC) stimulates tyrosine phosphorylation, activation of Sp1, Smad 3\/4 and c\u2010Myb, and production of collagen in lung fibroblasts","volume":"46","author":"Atamas SP","year":"2002","journal-title":"Arthritis Rheum"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-510X(02)00129-6"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65128-0"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.11164","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.11164","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T20:44:41Z","timestamp":1698698681000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.11164"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,7]]},"references-count":34,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2003,7]]}},"alternative-id":["10.1002\/art.11164"],"URL":"https:\/\/doi.org\/10.1002\/art.11164","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,7]]}}}