{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T04:31:15Z","timestamp":1773721875353,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2003,10,7]],"date-time":"2003-10-07T00:00:00Z","timestamp":1065484800000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Science, Sports, and Culture of Japan","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Kitasato University Graduate School of Medical Sciences"},{"name":"Ministry of Health, Labor, and Welfare of Japan"},{"name":"Kitasato University School of Medicine"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2003,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Hematopoietic prostaglandin D synthase (H\u2010PGDS) is a key enzyme in the production of prostaglandin D and its J series metabolites. We evaluated the antiinflammatory effect of retrovirally transfected H\u2010PGDS in order to investigate the role of H\u2010PGDS in monosodium urate monohydrate (MSU) crystal\u2013induced acute inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Expression of endogenous PGDS in a murine air\u2010pouch model of MSU crystal\u2013induced acute inflammation was determined by real\u2010time polymerase chain reaction. H\u2010PGDS complementary DNA (cDNA) was retrovirally transfected into C57BL\/6J fibroblasts, and the cells were designated as C57\u2010PGDS cells. Production of prostaglandins by C57\u2010PGDS cells was measured by enzyme immunoassay. The effect of C57\u2010PGDS cells on crystal\u2010induced inflammation was investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Injection of the crystals caused a rapid decrease in H\u2010PGDS expression by infiltrating cells and by the soft tissues around the air pouches. In contrast, expression of interleukin\u20101\u03b2 (IL\u20101\u03b2) and macrophage inflammatory protein 2 (MIP\u20102) as well as cellular infiltration were significantly increased during the early stage of inflammation. C57\u2010PGDS cells, but not control cells, produced an increased amount of PGD<jats:sub>2<\/jats:sub> in vitro, but suppressed production of PGE<jats:sub>2<\/jats:sub>. Injection of C57\u2010PGDS cells into air pouches inhibited cellular infiltration and MIP\u20102 and IL\u20101\u03b2 expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In this murine air\u2010pouch model of MSU crystal\u2013induced inflammation, retrovirally transfected H\u2010PGDS cDNA could reduce cellular infiltration, at least partly by inhibiting MIP\u20102 and IL\u20101\u03b2. These findings suggest that gene therapy with H\u2010PGDS may be useful for treating inflammatory diseases.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.11271","type":"journal-article","created":{"date-parts":[[2004,6,2]],"date-time":"2004-06-02T14:21:02Z","timestamp":1086186062000},"page":"2931-2941","source":"Crossref","is-referenced-by-count":61,"title":["Inhibition of monosodium urate monohydrate crystal\u2013induced acute inflammation by retrovirally transfected prostaglandin D synthase"],"prefix":"10.1002","volume":"48","author":[{"given":"Yousuke","family":"Murakami","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tohru","family":"Akahoshi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Izumi","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirahito","family":"Endo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsushi","family":"Hashimoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shizuka","family":"Kono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirobumi","family":"Kondo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinichi","family":"Kawai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matsuhisa","family":"Inoue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidero","family":"Kitasato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,10,7]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-68382-4_7"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115142"},{"key":"e_1_2_6_4_2","doi-asserted-by":"crossref","first-page":"3213","DOI":"10.4049\/jimmunol.138.10.3213","article-title":"Interleukin\u20101 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