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CTX\u2010II was quantified by competition enzyme\u2010linked immunosorbent assay (ELISA) based on a monoclonal antibody that recognized the C\u2010terminus of the peptide EKGPDP as a proteolytic neoepitope. Aggrecan fragments, matrix metalloproteinases 1 and 3, and tissue inhibitor of metalloproteinases 1 were determined by ELISAs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Concentrations of CTX\u2010II in SF were higher in patients with PPA, INJ, and POA than in the REF group (<jats:italic>P<\/jats:italic> &lt; 0.001). After joint injury, mean levels of CTX\u2010II in SF were increased above REF levels at all time intervals (<jats:italic>P<\/jats:italic> &lt; 0.001), and were highest within hours after trauma. In those in the longitudinal study group with joint cartilage damage, variation coefficients for CTX\u2010II were 81% (between patients) and 64% (within patient), monitored over 1 year. In a patient with septic arthritis, SF CTX\u2010II increased at the onset of symptoms, and peaked 30\u2010fold higher than the baseline. Concentrations of all biomarkers decreased with successful treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This is the first report to describe the release into SF of soluble molecular fragments specific for the degradation of mature, crosslinked, type II collagen (CII) in human OA and joint injury. The results provide strong evidence that the integrity of the CII network of cartilage is compromised soon after joint injury and in arthritis. This early degradation of CII may represent an important treatment target.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.11326","type":"journal-article","created":{"date-parts":[[2003,11,7]],"date-time":"2003-11-07T10:01:05Z","timestamp":1068199265000},"page":"3130-3139","source":"Crossref","is-referenced-by-count":216,"title":["The release of crosslinked peptides from type II collagen into human synovial fluid is increased soon after joint injury and in osteoarthritis"],"prefix":"10.1002","volume":"48","author":[{"given":"L. 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