{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T10:13:56Z","timestamp":1768904036159,"version":"3.49.0"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,11,22]],"date-time":"2005-11-22T00:00:00Z","timestamp":1132617600000},"content-version":"vor","delay-in-days":10552,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1977,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Glucocorticosteroids and mineralocorticosteroids were tested for their capacity to inhibit the nonphagocytic discharge of two lysosomal enzymes\u2014a cartilage matrix\u2010degrading neutral protease and \u03b2\u2010glucuronidase\u2014from highly purified human neutrophils. Lysosomal enzyme discharge from neutrophils adherent to nonphagocytizable, immobilized, heat\u2010aggregated IgG was inhibited by the four glucocorticosteroids\u2014methylprednisolone sodium succinate, triamcinolone acetonide hemisuccinate, para\u2010methasone acetate, and hydrocortisone sodium succinate. These glucocorticoids also inhibited zymosan\u2010induced release of \u03b2\u2010glucuronidase from neutrophils that had been pretreated with cytochalasin B in order to completely prevent the onset of phagocytosis. Inhibition by the glucocorticoids of lysosomal enzyme discharge provoked by a soluble divalent cation ionophore was also observed. Neither desoxycorticosterone acetate nor aldosterone hemisuccinate, two mineralocorticosteroids, inhibited lysosomal enzyme release. Similarly, the salt moieties of some of the steroids tested, such as sodium succinate and sodium acetate, failed to elicit an effect on enzyme release. Therefore interference with lysosomal enzyme discharge was restricted to the glucocorticosteroid ring structure. Because interference either with the adherence of neutrophils to immune reactants or with the activities of the discharged lysosomal enzymes by the glucocorticoids could be interpreted as inhibition of lysosomal enzyme release, steroidal effects on these parameters were examined. None of the glucocorticoids tested elicited any significant effects on neutrophil adherence or lysosomal enzyme activity. Thus it appears that glucocorticosteroids are capable of inhibiting directly the nonphagocytic discharge of lysosomal enzymes from human neutrophils.<\/jats:p>","DOI":"10.1002\/art.1780200114","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T18:06:42Z","timestamp":1171476402000},"page":"73-83","source":"Crossref","is-referenced-by-count":26,"title":["Glucocorticosteroid inhibition of nonphagocytic discharge of lysosomal enzymes from human neutrophils"],"prefix":"10.1002","volume":"20","author":[{"given":"Louis J.","family":"Ignarro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(61)90328-7"},{"key":"e_1_2_1_3_2","first-page":"215","article-title":"The effects of corticosteroids upon connective tissue and lysosomes","volume":"20","author":"Weissmann G","year":"1964","journal-title":"Recent Prog Horm Res"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(71)90196-1"},{"key":"e_1_2_1_5_2","first-page":"179","article-title":"Lysosome membrane stabilization in vivo: effects of steroidal and nonsteroidal antiinflammatory drugs on the integrity of rat liver lysosomes","volume":"182","author":"Ignarro LJ","year":"1972","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/newbio239155a0"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.111.5.705"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj0790500"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj0870403"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.124.4.733"},{"key":"e_1_2_1_11_2","first-page":"665","article-title":"Glomerular basement membrane damage in immunological glomerulonephritis","volume":"14","author":"Hawkins D","year":"1968","journal-title":"Immunology"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(72)90131-1"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(73)90034-2"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.128.5.1137"},{"key":"e_1_2_1_15_2","first-page":"228","article-title":"Mediators of tissue damage in leukocyte lysosomes. 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Further studies on human granulocyte elastase","volume":"22","author":"Janoff A","year":"1970","journal-title":"Lab Invest"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780110206"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780180413"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01965227"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780160608"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(74)90034-8"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780170108"},{"key":"e_1_2_1_22_2","doi-asserted-by":"crossref","first-page":"298","DOI":"10.4049\/jimmunol.113.1.298","article-title":"Release of neutral protease and \u03b2\u2010glucuronidase from human neutrophils in the presence of cartilage treated with various immunologic reactants","volume":"113","author":"Ignarro LJ","year":"1974","journal-title":"J Immunol"},{"key":"e_1_2_1_23_2","first-page":"283","article-title":"Lysosomal enzyme secretion from human neutrophils mediated by cyclic GMP: inhibition of cyclic GMP accumulation and neutrophil function by glucocorticosteroids","volume":"1","author":"Ignarro LJ","year":"1975","journal-title":"J Cyclic Nucl Res"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.72.1.108"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.139.6.1395"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.138.2.461"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780170106"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.134.3.149"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.70.3.844"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI105945"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/246417a0"},{"key":"e_1_2_1_32_2","doi-asserted-by":"crossref","first-page":"1535","DOI":"10.4049\/jimmunol.107.6.1535","article-title":"The immunologic release of constituents from neutrophil leukocytes. 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