{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:30:59Z","timestamp":1759332659389},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,11,23]],"date-time":"2005-11-23T00:00:00Z","timestamp":1132704000000},"content-version":"vor","delay-in-days":9458,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1980,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Using a sensitive <jats:sup>125<\/jats:sup>I\u2010protein A (PrA) binding assay to detect cell surface IgG, we have studied seven different synovial fibroblast cell cultures from patients with rheumatoid arthritis (RA). When these cultures were incubated in the presence of serum from 18 autologous and allogeneic RA patients (all seropositive), we were unable to detect significant IgG binding. Since IgM rheumatoid factor (RF) can block PrA binding, sera were absorbed with aggregated IgG to remove RF without affecting the results. Similar studies on three cell lines with seven rheumatoid sera were performed by antibody\u2010dependent cell\u2010mediated cytotoxicity. No significant cytotoxicity was observed. Since antibodies to collagen are present in rheumatoid sera, several cultures were incubated with ascorbic acid (12.5 \u03bcg\/ml) to optimize synthesis of cell surface collagen. These culture conditions did not affect serum immunoglobulin binding by the <jats:sup>125<\/jats:sup>I\u2010PrA assay. Thus, we can find no evidence for a direct humoral immune mediation of synovial proliferation in rheumatoid arthritis. These data do not support the hypothesis that the inflammatory process within the synovium of RA patients is an immunologic response to a fibroblast\u2010associated antigen in the synovial membrane.<\/jats:p>","DOI":"10.1002\/art.1780230103","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T18:41:01Z","timestamp":1171478461000},"page":"10-16","source":"Crossref","is-referenced-by-count":8,"title":["Studies of serum immunoglobulin binding to synovial fibroblast cell cultures from patients with rheumatoid arthritis"],"prefix":"10.1002","volume":"23","author":[{"given":"William B.","family":"Gruhn","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frederic C.","family":"Mcduffie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60299-0"},{"key":"e_1_2_1_3_2","first-page":"131","article-title":"Pathophysiology of rheumatoid arthritis","volume":"32","author":"Ziff M","year":"1973","journal-title":"Fed Proc"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1975.tb36041.x"},{"key":"e_1_2_1_5_2","first-page":"245","volume-title":"A microbiologist's view of investigative rheumatology, Infection and Immunology in the Rheumatic Diseases","author":"Marmion BP","year":"1976"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1975.tb36033.x"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780210116"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780210213"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780090405"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60299-0"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780190111"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"822","DOI":"10.4049\/jimmunol.97.6.822","article-title":"\u201cProtein A\u201d from S. aureus. 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