{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:03:11Z","timestamp":1762160591368},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,11,23]],"date-time":"2005-11-23T00:00:00Z","timestamp":1132704000000},"content-version":"vor","delay-in-days":9398,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1980,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A solid\u2010phase radioimmunoassay capable of detecting nanogram quantities of human IgM rheumatoid factor (RF) in biologic fluids has been developed. Binding curves for monoclonal IgM RF and polyclonal IgM rheumatoid factors were similar under the conditions utilized for the assay. Human IgG did not interfere with the detection of IgM RF by this method. Small quantities (\u2264 0.2%) of nonspecific binding by nonRF IgM to the human IgG coated tubes utilized in the assay were corrected for by assaying samples in parallel bovine serum albumin coated control tubes. As expected, patients with seropositive rheumatoid arthritis (RA) had significantly higher concentrations of IgM RF than seronegative RA patients (mean \u00b1 1 SD = 652 \u00b1 553 \u03bcg\/ml versus 11.3 \u00b1 13.3 \u03bcg\/ml, <jats:italic>P<\/jats:italic> &lt; 0.001). In contrast, all normal control sera assayed to date contained &lt; 0.1 \u03bcg\/ml of IgM RF. The capacity of the assay to detect nanogram quantities of IgM RF should permit investigation of the cellular mechanisms underlying RF production.<\/jats:p>","DOI":"10.1002\/art.1780230306","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T19:06:16Z","timestamp":1171479976000},"page":"302-308","source":"Crossref","is-referenced-by-count":51,"title":["A sensitive radioimmunoassay for quantitation of igm rheumatoid factor"],"prefix":"10.1002","volume":"23","author":[{"given":"William J.","family":"Koopman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralph E.","family":"Schrohenloher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,23]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"504","volume-title":"Rheumatoid factors, Arthritis and Allied Conditions","author":"Mannik M","year":"1979"},{"key":"e_1_2_1_3_2","first-page":"541","volume-title":"Rheumatoid and sero\u2010nega\u2010tive arthritides, Immunology in Medicine","author":"Holborow EJ","year":"1977"},{"key":"e_1_2_1_4_2","first-page":"351","volume-title":"Autoantibodies and disease, Advances in Immunology","author":"Kunkel HG","year":"1964"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"365","DOI":"10.4049\/jimmunol.115.2.365","article-title":"The molecular basis of self\u2010association of IgG\u2010rheumatoid factors","volume":"115","author":"Pope RM","year":"1975","journal-title":"J Immunol"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780170312"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI105758"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI108546"},{"key":"e_1_2_1_9_2","first-page":"197","article-title":"Rheumatoid factor plaque\u2010forming cells in rheumatoid synovial tissue","volume":"28","author":"Taylor\u2010Upsahl MM","year":"1977","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780210713"},{"key":"e_1_2_1_11_2","first-page":"647","article-title":"Induction of human antiimmunoglobulin antibody (rheumatoid factor) producing cells by aggregated IgG (abstract)","volume":"22","author":"Pisko EJ","year":"1979","journal-title":"Arthritis Rheum"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)63022-4"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.112.1.203"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.120.6.1215"},{"key":"e_1_2_1_15_2","first-page":"16","article-title":"Revision of diagnostic criteria of rheumatoid arthritis","volume":"2","author":"A Committee of the American Rheumatism Association","year":"1958","journal-title":"Arthritis Rheum"},{"key":"e_1_2_1_16_2","first-page":"888","article-title":"The latex fixation test. 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