{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:33:43Z","timestamp":1760888023147},"reference-count":26,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2005,11,23]],"date-time":"2005-11-23T00:00:00Z","timestamp":1132704000000},"content-version":"vor","delay-in-days":9214,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1980,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Peripheral blood mononuclear leukocytes (MNL) from 42 patients with classic\/definite seropositive rheumatoid arthritis (RA) and 24 healthy adult controls were tested for their capacity to produce IgM rheumatoid factor (RF) in vitro in the presence and absence of pokeweed mitogen (PWM). In no instance was spontaneous elaboration of IgM RF from control MNL observed. In contrast, MNL from 16 of the 42 RA patients spontaneously synthesized IgM RF (22 \u00b1 43 ng\/ culture) which constituted a substantial fraction of the total IgM in these culture fluids (48 \u00b1 26%). Pokeweed mitogen induced detectable quantities of IgM RF in MNL culture supernatants from 10 of 24 controls (12 \u00b1 11 ng\/culture) and 33 of 42 RA patients (60 \u00b1 82 ng\/ culture, <jats:italic>p<\/jats:italic> = 0.008). IgM RF constituted a significantly higher proportion of the total IgM in RA MNL supernatants than in control supernatants (11 \u00b1 11% versus 1.01 \u00b1 1.03%; <jats:italic>P<\/jats:italic> = 0.013). IgM RF production (spontaneous and PWM\u2010induced) was dependent upon de novo protein synthesis. The results indicate that B cells programmed to produce IgM RF are present in both normal and RA B cell repertoires, but are preferentially expressed in RA.<\/jats:p>","DOI":"10.1002\/art.1780230904","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T18:51:00Z","timestamp":1171479060000},"page":"985-992","source":"Crossref","is-referenced-by-count":56,"title":["Enhanced in vitro synthesis of igm rheumatoid factor in rheumatoid arthritis"],"prefix":"10.1002","volume":"23","author":[{"given":"William J.","family":"Koopman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralph E.","family":"Schrohenloher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,23]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"504","volume-title":"Rheumatoid factors, Arthritis and Allied Conditions","author":"Mannik M","year":"1979"},{"key":"e_1_2_1_3_2","first-page":"541","volume-title":"Rheumatoid and seronegative arthritides, Immunology in Medicine","author":"Holborow EJ","year":"1977"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.105.5.425"},{"key":"e_1_2_1_5_2","first-page":"689","article-title":"Gamma globulin complexes in synovial fluids of patients with rheumatoid arthritis. 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