{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T11:34:07Z","timestamp":1770982447918,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,11,23]],"date-time":"2005-11-23T00:00:00Z","timestamp":1132704000000},"content-version":"vor","delay-in-days":9033,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1981,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recently, antibodies reactive with T cell subpopulations have been reported to exist in children with active juvenile arthritis (JRA). In an attempt to verify and extend these observations, we have studied children with JRA for the presence of anti\u2010T cell antibodies by flow cytometry and cytoadherence rosette techniques. T cells were isolated from peripheral blood mononuclear cells (PBL) by two methods: 1) Differential sedimentation of PBL rosetted with neuraminidase\u2010treated sheep erythrocytes, and 2) removal of immunoglobulin positive PBL by rosetting with rabbit anti\u2013human F(ab\u2032)<jats:sub>2<\/jats:sub> coated bovine erythrocytes and differential sedimentation. Utilizing these methods to detect lymphoreactivity of JRA sera to either population of T cell isolates, we observed the binding of ultracentrifuged normal human sera (NHS) to be comparable to JRA sera (active and quiescent). NHS reacted with 15\u201325% of T cells. Further studies demonstrate that monomeric IgG was chiefly responsible for lymphoreactivity. The results of these studies are discussed in the context of previous observations.<\/jats:p>","DOI":"10.1002\/art.1780240302","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T19:20:57Z","timestamp":1171480857000},"page":"457-463","source":"Crossref","is-referenced-by-count":8,"title":["Flow cytometry and cytoadherence studies of sera from children with juvenile rheumatoid arthritis and normal controls"],"prefix":"10.1002","volume":"24","author":[{"given":"Christopher J.","family":"Froelich","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arthur D.","family":"Bankhurst","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William E.","family":"Crowe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Ralph C.","family":"Williams","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noel L.","family":"Warner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph E.","family":"Levinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1968.0032"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.141.6.1376"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.141.6.1390"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.147.2.446"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4684-3054-7_10"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.146.1.184"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"65","DOI":"10.4049\/jimmunol.122.1.65","article-title":"A receptor for IgA on human T lymphocytes","volume":"122","author":"Lum LG","year":"1979","journal-title":"J Immunol"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"2577","DOI":"10.4049\/jimmunol.122.6.2577","article-title":"Lymphocytes bearing Fc receptors for IgE. 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Presence of human and rat T lymphocytes with Fc receptors","volume":"122","author":"Yodoi J","year":"1979","journal-title":"J Immunol"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"1272","DOI":"10.4049\/jimmunol.123.3.1272","article-title":"Markers of T cell subsets identified by alloantisera","volume":"123","author":"Ferrara GB","year":"1979","journal-title":"J Immunol"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.4049\/jimmunol.120.4.1278","article-title":"Isolation and characterization of naturally occurring subclasses of human peripheral blood T cells with regulatory functions","volume":"120","author":"Strelkauskas AJ","year":"1978","journal-title":"J Immunol"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.10.5150"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(79)90124-7"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.146.6.1719"},{"key":"e_1_2_1_15_2","first-page":"62","article-title":"Enumeration and isolation of human T and B lymphocytes by rosette formation with antibody\u2010coated erythrocytes","volume":"22","author":"Strelkauskas AJ","year":"1975","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_1_16_2","doi-asserted-by":"crossref","first-page":"859","DOI":"10.4049\/jimmunol.99.5.859","article-title":"Chromic chloride: a coupling reagent for passive hemagglutination reactions","volume":"99","author":"Gold ER","year":"1967","journal-title":"J Immunol"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1063\/1.1685647"},{"key":"e_1_2_1_18_2","first-page":"224","volume-title":"Methods in Immunology","author":"Campbell DH","year":"1970"},{"key":"e_1_2_1_19_2","first-page":"593","article-title":"Studies of lymphocytotoxins in infectious mononucleosis and systemic lupus erythematosus: evidence for immune complex\u2010mediated cytotoxicity","volume":"39","author":"Quin JW","year":"1980","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780200807"},{"key":"e_1_2_1_21_2","first-page":"126","article-title":"Comparison of various tests for Fc receptors on different human lymphocyte subpopulations","volume":"37","author":"Winchester RJ","year":"1979","journal-title":"Clin Exp Immunol"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.1780240302","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.1780240302","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T20:02:59Z","timestamp":1700769779000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.1780240302"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,3]]},"references-count":20,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1981,3]]}},"alternative-id":["10.1002\/art.1780240302"],"URL":"https:\/\/doi.org\/10.1002\/art.1780240302","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[1981,3]]}}}