{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T22:14:48Z","timestamp":1757542488212},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,11,23]],"date-time":"2005-11-23T00:00:00Z","timestamp":1132704000000},"content-version":"vor","delay-in-days":9033,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1981,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A sensitive solid phase microradioimmu\u2010noassay has been developed for measurement of antidouble stranded DNA (dsDNA) antibodies. In this procedure, advantage has been taken of the capacity of poly\u2010L\u2010lysine (PLL) to facilitate the binding of pure dsDNA to plastic surfaces. In the absence of PLL, binding did not occur. Diluted sera were incubated in PLL\u2010treated dsDNA\u2010coated microtitration trays and anti\u2010dsDNA Ig was measured using affinity purified<jats:sup>125<\/jats:sup>I\u2010anti\u2010Ig of high specific activity. The synthetic DNA, poly dA\u2010dT, was used as a model for dsDNA. In initial experiments, specific anti\u2010DNA binding could not be demonstrated because of high background binding of patient Ig to PLL\u2010treated surfaces. This was reduced by diluting test sera and anti\u2010Ig in buffer containing 2% BGG and 1% BSA. Specificity of the assay for DNA was demonstrated by absorbing the anti\u2010DNA activity on DNA\u2010coated plastic. The binding of systemic lupus erythema\u2010tosus (SLE) patient serum Ig to poly dA\u2010dT coated trays did not diminish after digestion with nuclease S<jats:sup>1<\/jats:sup>, suggesting that the synthetic polymer is an appropriate model for dsDNA. Patient and normal sera were screened for anti\u2010dsDNA activity using poly dA\u2010dT as antigen. None of the 38 normal sera, 23 of 35 active SLE sera, 1 of 25 treated SLE, 4 of 35 rheumatoid arthritis, 3 of 35 scleroderma, and 1 of 13 polymyositis sera demonstrated positive anti\u2010dsDNA activity. The anti\u2010dsDNA values obtained in the radioimmunoassay correlated significantly with those obtained in the <jats:italic>Crithidia luciliae<\/jats:italic> assay.<\/jats:p>","DOI":"10.1002\/art.1780240314","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T19:20:57Z","timestamp":1171480857000},"page":"534-543","source":"Crossref","is-referenced-by-count":39,"title":["A sensitive solid phase microradioimmunoassay for anti\u2010double stranded DNA 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