{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T03:34:15Z","timestamp":1790220855615,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,11,28]],"date-time":"2005-11-28T00:00:00Z","timestamp":1133136000000},"content-version":"vor","delay-in-days":8946,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1981,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Siderosis of rabbit articular chondrocytes was produced in vitro as a model for the cartilage damage of hemophilic arthropathy. Both FeSO<jats:sub>4<\/jats:sub> 0.1\u20132.5 m<jats:italic>M<\/jats:italic> and rabbit hemoglobin (as hemolyzed serum, 14 mg\/ml) caused iron storage in cell and organ culture. FeSO<jats:sub>4<\/jats:sub> was far more effective. The fine structure of the siderosomes resulting from both iron sources was comparable to that observed in hemophilic and other forms of hemosiderosis. Particles resembling ferric oxyhydroxide were included in the FeSO<jats:sub>4<\/jats:sub> but not the hemoglobin derived siderin. Iron storage following FeSO<jats:sub>4<\/jats:sub> was enhanced 5\u2010fold by culturing with rabbit rather than fetal calf serum. Despite repeated washing of the cultures and detachment with trypsin, an extracellular pool of Fe<jats:sup>3+<\/jats:sup> persisted in the cell pellets. Cytotoxicity of Fe was manifested by formation of myelin bodies and a dose\u2010dependent reduction of cell number. There was an inverse relationship betweeen cytotoxicity and iron storage following administration of FeSO<jats:sub>4<\/jats:sub> to five other cell types. Ascorbate 40 \u03bcg\/ml stimulated DNA synthesis but had no protective effect against the cytotoxicity of FeSO<jats:sub>4<\/jats:sub>. Little erythrophagocytosis was shown by the chondrocytes. Desferrioxamine (0.01\u20132.5 m<jats:italic>M<\/jats:italic>) was markedly toxic for dividing but not for stationary chondrocytes. Administered after iron storage had been induced with FeSO<jats:sub>4<\/jats:sub>, 1.0\u20132.5 m<jats:italic>M<\/jats:italic> desferrioxamine removed stainable siderin granules over the course of 4 days.<\/jats:p>","DOI":"10.1002\/art.1780240609","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T19:15:23Z","timestamp":1171480523000},"page":"809-823","source":"Crossref","is-referenced-by-count":21,"title":["Experimental siderosis of articular chondrocytes cultured in vitro"],"prefix":"10.1002","volume":"24","author":[{"given":"Ye\u2010Chin","family":"Choi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aubrey J.","family":"Hough","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory M.","family":"Morris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leon","family":"Sokoloff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,11,28]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780210122"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-197759030-00001"},{"key":"e_1_2_1_4_2","first-page":"955","article-title":"L\u00e9sions articulaires de l'h\u00e9mophilie","volume":"49","author":"Amouroux J","year":"1973","journal-title":"Sem Hop Paris"},{"key":"e_1_2_1_5_2","first-page":"199","article-title":"On the pathology of haemophilic joints","volume":"9","author":"Storti E","year":"1974","journal-title":"Pathol Europ"},{"key":"e_1_2_1_6_2","first-page":"91","article-title":"Cartilage in hemophilic arthropathy:ultrastructural and microanalytical studies","volume":"100","author":"Hough AJ","year":"1976","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_1_7_2","first-page":"345","article-title":"Articular cartilage degradation and the pathology of haemophilic arthropathy","volume":"54","author":"Rippey JJ","year":"1978","journal-title":"S Afr Med J"},{"key":"e_1_2_1_8_2","first-page":"264","article-title":"\u00c8tude histologique et histochimique des l\u00e9sions articulaires de la chondrocalcinose survenant au cours d'une h\u00e9mochromatose","volume":"14","author":"Mitrovic D","year":"1966","journal-title":"Arch Anat Pathol"},{"key":"e_1_2_1_9_2","first-page":"69","article-title":"Ultrastructure of articular cartilage in experimental hemarthrosis","volume":"86","author":"Roy S","year":"1968","journal-title":"Arch Pathol"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/BF02894062","article-title":"Electron probe x\u2010ray analysis of siderosomes in hemarthrotic articular cartilage","volume":"16","author":"Ghadially FN","year":"1974","journal-title":"Virchows Arch Zellpath"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.141.3585.1055"},{"key":"e_1_2_1_12_2","first-page":"55","volume-title":"Clinical Disorders of Iron Metabolism","author":"Fairbanks VF","year":"1971"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780130203"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003086-197103000-00030"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.3109\/03008207809152628"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-90-1-262"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.26.3.251"},{"key":"e_1_2_1_18_2","first-page":"71","article-title":"Recommendations for haemoglobinometry in human blood","volume":"13","author":"International Committee for Standardization in Haematology","year":"1967","journal-title":"Br J Haematol"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(73)90309-6"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2423(08)60414-X"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj0620315"},{"key":"e_1_2_1_22_2","volume-title":"Histophatologic Technique and Practical Histochemistry","author":"Lillie RD","year":"1965"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.3109\/03008208009152295"},{"key":"e_1_2_1_24_2","first-page":"271","article-title":"Haemorrhage and haemosiderin","volume":"11","author":"Ghadially FN","year":"1979","journal-title":"J Submicro Cytol"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(70)86307-X"},{"key":"e_1_2_1_26_2","first-page":"363","article-title":"The iron\u2010loaded cell:the cytopathology of iron storage","volume":"91","author":"Richter GW","year":"1978","journal-title":"Am J Pathol"},{"key":"e_1_2_1_27_2","first-page":"73","volume-title":"Ferritin and Haemosiderin: structure and function, Iron Biochemistry and Medicine","author":"Harrison PM","year":"1974"},{"key":"e_1_2_1_28_2","first-page":"427","volume-title":"Non\u2010transferrin plasma iron in patients with transfusional iron overload, Proteins of Iron Storage & Transport in Biochemistry and Medicine","author":"Hershko C","year":"1976"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0028-1113001"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1969.tb00666.x"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(73)90594-6"},{"key":"e_1_2_1_32_2","first-page":"295","article-title":"The relationship of intracellular pathways of iron metabolism to cellular iron overload and the iron storage diseases","volume":"72","author":"Trump BF","year":"1973","journal-title":"Am J Pathol"},{"key":"e_1_2_1_33_2","first-page":"33","article-title":"Iron metabolism and cell membranes. III. Iron\u2010induced alterations in HeLa cells","volume":"80","author":"Jauregui HO","year":"1975","journal-title":"Am J Pathol"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(78)90066-1"},{"key":"e_1_2_1_35_2","first-page":"8","article-title":"Pr\u00e4arthrose und pr\u00e4arthrotische Deformit\u00e4t:Eine Studie zur Erweilung des Begriffs von Hackenbroch","volume":"112","author":"Cotta H","year":"1974","journal-title":"Z Orthop"},{"key":"e_1_2_1_36_2","first-page":"118","article-title":"Synovektomie und Gelenkknorpel","volume":"112","author":"Schulitz KP","year":"1974","journal-title":"Z Orthop"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.46.070177.003321"},{"key":"e_1_2_1_38_2","first-page":"317","article-title":"Microhemorrhage in the hyperplastic thyroid gland","volume":"85","author":"Zeligs JD","year":"1976","journal-title":"Am J Pathol"},{"key":"e_1_2_1_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00225361"},{"key":"e_1_2_1_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(79)90100-0"},{"key":"e_1_2_1_41_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1975.tb01857.x"},{"key":"e_1_2_1_42_2","first-page":"37","volume-title":"New iron chelators, Chelation Therapy in Chronic Iron Overload","author":"Cerami A","year":"1979"},{"key":"e_1_2_1_43_2","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1182\/blood.V40.6.812.812","article-title":"Hemoglobin and erythrocyte catabolism in rat liver:the separate roles of parenchymal and sinusoidal cells","volume":"40","author":"Bissell DM","year":"1972","journal-title":"Blood"},{"key":"e_1_2_1_44_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.69.12.3708"},{"key":"e_1_2_1_45_2","first-page":"76","volume-title":"Isolation of microbial iron chelators: some possible effects of their chemotherapeutic use, Symposium on Development of Iron Chelators for Clinical Use","author":"Byers BR","year":"1975"},{"key":"e_1_2_1_46_2","first-page":"358","article-title":"The use of Chang liver cells cultured in vitro for the investigation of cellular iron metabolism","volume":"56","author":"Bailey\u2010Wood R","year":"1975","journal-title":"Br J Exp Pathol"},{"key":"e_1_2_1_47_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1979.tb03682.x"},{"key":"e_1_2_1_48_2","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1182\/blood.V48.6.923.923","article-title":"The effect of chelating agents on iron metabolism in Chang cell cultures","volume":"48","author":"White GP","year":"1976","journal-title":"Blood"},{"key":"e_1_2_1_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(77)91508-X"},{"key":"e_1_2_1_50_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1971.tb07024.x"},{"key":"e_1_2_1_51_2","unstructured":"KrystalG Morris GM SokoloffL:Ascorbate stimulates DNA synthesis by cultured articular chondrocytes. Submitted for publication"},{"key":"e_1_2_1_52_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780190110"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.1780240609","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.1780240609","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T17:57:32Z","timestamp":1700762252000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.1780240609"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,6]]},"references-count":51,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1981,6]]}},"alternative-id":["10.1002\/art.1780240609"],"URL":"https:\/\/doi.org\/10.1002\/art.1780240609","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[1981,6]]}}}