{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T11:33:32Z","timestamp":1770982412673,"version":"3.50.1"},"reference-count":14,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,11,28]],"date-time":"2005-11-28T00:00:00Z","timestamp":1133136000000},"content-version":"vor","delay-in-days":8336,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1983,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sera from 10 patients with juvenile arthritis (JA), 2 seropositive and 8 with hidden rheumatoid factor (RF), were subjected to affinity chromatography on a rabbit anti\u2010human IgM column. Material retained by the column was eluted sequentially by 1<jats:italic>M<\/jats:italic> NH<jats:sub>3<\/jats:sub> and 0.1<jats:italic>M<\/jats:italic> glycine\u2010HCl buffer, pH 3.0. The affinity fractions contained both 19S IgM RF and IgG, while corresponding fractions from healthy controls contained neither. Sera from 15 patients with JA, 1 seropositive and 11 with hidden RF, were subjected to 4% polyethylene glycol precipitation followed by acid dissociation of the precipitate. Ten of 15 resultant fractions contained both IgM RF and IgG, while corresponding fractions from healthy controls contained only traces of IgG. Sera from 7 of these JA patients were subjected to sucrose density gradient centrifugation and the resultant fractions analyzed for the presence of immune complexes by the Clq solid\u2010phase assay. Immune complexes were detected at and ahead of the IgM marker, as expected for IgM RF\u2010IgG complexes. These combined data show that the majority of JA patients with classic or hidden 19S IgM RF have immune complexes containing IgM RF and IgG in their sera.<\/jats:p>","DOI":"10.1002\/art.1780260207","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T19:53:43Z","timestamp":1171482823000},"page":"165-169","source":"Crossref","is-referenced-by-count":13,"title":["Separation and characterization of immune complexes containing 19S IgM rheumatoid factor\u2010IgG in juvenile arthritis"],"prefix":"10.1002","volume":"26","author":[{"given":"Terry L.","family":"Moore","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Wayne Sheridan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jack","family":"Zuckner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert W.","family":"Dorner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,28]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"25","article-title":"Immunologic studies in juvenile arthritis","volume":"32","author":"Moore TL","year":"1982","journal-title":"Bull Rheum Dis"},{"key":"e_1_2_1_3_2","first-page":"665","article-title":"Clq binding in serum in juvenile rheumatoid arthritis","volume":"7","author":"Miller JJ","year":"1980","journal-title":"J Rheumatol"},{"key":"e_1_2_1_4_2","first-page":"395","article-title":"Immune complexes in juvenile rheumatoid arthritis: a comparison of four methods","volume":"9","author":"Moore TL","year":"1982","journal-title":"J Rheumatol"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780210810"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-198010000-00010"},{"key":"e_1_2_1_7_2","first-page":"195","article-title":"Current proposed revision of JRA criteria: JRA Criteria Subcommittee of the Diagnostic and Therapeutic Criteria Committee of The American Rheumatism Association Section of The Arthritis Foundation","volume":"20","author":"Brewer EJ","year":"1977","journal-title":"Arthritis Rheum"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(80)90176-3"},{"key":"e_1_2_1_9_2","first-page":"399","article-title":"Quantitation and characterization of soluble immune complexes precipitated from sera by polyethylene glycol","volume":"37","author":"Chia D","year":"1979","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_1_10_2","first-page":"615","article-title":"Study of circulating immune complex size in systemic lupus erythematosus","volume":"43","author":"Tung KSK","year":"1981","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"295","DOI":"10.4049\/jimmunol.119.1.295","article-title":"Radioimmunoassay of IgG and IgM rheumatoid factors reacting with human IgG","volume":"149","author":"Carson DA","year":"1977","journal-title":"J Immunol"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780241008"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.39.2.129"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01964384"},{"key":"e_1_2_1_15_2","first-page":"599","article-title":"Longitudinal study of the presence of hidden 19S IgM rheumatoid factor in juvenile rheumatoid arthritis","volume":"9","author":"Moore TL","year":"1982","journal-title":"J Rheumatol"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.1780260207","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.1780260207","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T23:46:19Z","timestamp":1700696779000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.1780260207"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,2]]},"references-count":14,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1983,2]]}},"alternative-id":["10.1002\/art.1780260207"],"URL":"https:\/\/doi.org\/10.1002\/art.1780260207","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[1983,2]]}}}