{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T20:59:19Z","timestamp":1761512359570},"reference-count":39,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2005,11,28]],"date-time":"2005-11-28T00:00:00Z","timestamp":1133136000000},"content-version":"vor","delay-in-days":8033,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1983,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>High levels of anti\u2010immunoglobulins that react with Fab\u2032 fragments of IgG have been observed in sera of patients with rheumatoid arthritis (RA). To determine how much of these anti\u2010Fab\u2032 antibodies are incorporated within immune complexes (IC), we added <jats:sup>125<\/jats:sup>I\u2010Fab\u2032 to sera and then measured the amount of labeled Fab\u2032 that could be precipitated by adding polyethylene glycol (PEG). Sera were either maintained at neutral pH during this procedure or acidified (pH 3) to dissociate IC. Acidification permitted the <jats:sup>125<\/jats:sup>I\u2010Fab\u2032 an equal chance to compete with other endogenous antigens for anti\u2010Fab\u2032 antibodies, once excess hydrogen ion was removed. Quantities of anti\u2010Fab\u2032 in sera of 20 seropositive RA patients were greater than in sera of 43 age\u2010and sex\u2010matched healthy controls, as measured by this assay and by a solid phase radioimmunoassay. However, significantly less anti\u2010Fab\u2032 antibody was incorporated in ICs in the RA patients' sera. Supernatants from cultured peripheral blood lymphocytes of RA patients also contained relatively more \u201cfree\u201d and less \u201chidden\u201d anti\u2010Fab\u2032 than culture supernatants from controls. Thus there appear to be qualitative as well as quantitative differences in the anti\u2010Fab\u2032 antibodies synthesized by RA patients. This may reflect different proportions of IgM and IgG anti\u2010Fab\u2032 in their sera, differences in the average avidity of these antibodies, or differences in the reciprocal relationships within the idiotypic network that result in release of antibodies by certain antibody\u2010producing cells, but not by clones that produce complementary idiotypes.<\/jats:p>","DOI":"10.1002\/art.1780261210","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T19:53:46Z","timestamp":1171482826000},"page":"1481-1492","source":"Crossref","is-referenced-by-count":30,"title":["Anti\u2010fab' antibodies in rheumatoid arthritis"],"prefix":"10.1002","volume":"26","author":[{"given":"Holly H.","family":"Birdsall","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin D.","family":"Lidsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger D.","family":"Rossen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,28]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"57","article-title":"Studies into the occurrence of soluble antigen\u2010antibody complexes in disease. 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