{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:20:37Z","timestamp":1760188837521},"reference-count":14,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,11,28]],"date-time":"2005-11-28T00:00:00Z","timestamp":1133136000000},"content-version":"vor","delay-in-days":8002,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1984,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Two distinct nuclear antigens, designated NSpI and NSpII, have been characterized and differentiated from the centromeric antigen that reacts with sera from patients with the CREST syndrome. Both NSpI and NSpII produce a speckled pattern of indirect immuno\u2010fluorescence on HEp\u20102 cells that resembles the pattern seen with anticentromere antibodies (ACA). They are differentiated from the ACA staining pattern by the absence of metaphase chromatin staining by NSpI antisera and by the absence of a discrete speckled pattern of staining by NSpII. Further, both NSpI and NSpII stain predominantly the peritubular nuclei of mouse kidney cryostat sections. NSpII is sensitive to trypsin, proteinase K, and HCl extraction, suggesting that it is a relatively soluble nuclear protein. NSpI was also sensitive to protease treatment but was not extracted with 0.1<jats:italic>N<\/jats:italic> HCl, suggesting that it is a tightly bound nuclear protein.<\/jats:p>","DOI":"10.1002\/art.1780270114","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T20:10:06Z","timestamp":1171483806000},"page":"92-96","source":"Crossref","is-referenced-by-count":42,"title":["Speckled pattern antinuclear antibodies resembling anticentromere antibodies"],"prefix":"10.1002","volume":"27","author":[{"given":"Marvin J.","family":"Fritzler","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Don W.","family":"Valencia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gale A.","family":"Mccarty","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,28]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.3.1627"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(80)90462-3"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780230602"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.41.4.382"},{"key":"e_1_2_1_6_2","first-page":"167A","article-title":"Anti\u2010centromere antibody (ACA): clinical and serologic correlates in 27 patients compared to diffuse scleroderma (PSS)","volume":"29","author":"McCarty GA","year":"1981","journal-title":"Clin Res"},{"key":"e_1_2_1_7_2","first-page":"603","article-title":"Studies of antinuclear antibodies in rheumatoid arthritis","volume":"9","author":"Garcia\u2010de la Torre I","year":"1982","journal-title":"J Rheumatol"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780260714"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.41.6.612"},{"key":"e_1_2_1_10_2","first-page":"607","article-title":"Anti\u2010centromere antibody and primary pulmonary hypertension","volume":"9","author":"Seibold JR","year":"1982","journal-title":"J Rheumatol"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI109161"},{"key":"e_1_2_1_12_2","first-page":"391","article-title":"The binding of anti\u2010DNA antibodies as measured fluorometrically by ethidium bromide","volume":"5","author":"Shepherd JD","year":"1978","journal-title":"J Rheumatol"},{"key":"e_1_2_1_13_2","first-page":"28B","article-title":"Species distribution and biochemical characterization of centromeric antigen that reacts with human autoimmune serum (abstract)","volume":"5","author":"Ayer LM","year":"1982","journal-title":"Clin Invest Med"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.121.6.2228"},{"key":"e_1_2_1_15_2","first-page":"S122","article-title":"SL\u2014a new precipitating system (abstract)","volume":"24","author":"Harmon C","year":"1981","journal-title":"Arthritis Rheum"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.1780270114","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.1780270114","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T00:54:40Z","timestamp":1697676880000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.1780270114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,1]]},"references-count":14,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1984,1]]}},"alternative-id":["10.1002\/art.1780270114"],"URL":"https:\/\/doi.org\/10.1002\/art.1780270114","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[1984,1]]}}}