{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T11:34:07Z","timestamp":1770982447939,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,11,28]],"date-time":"2005-11-28T00:00:00Z","timestamp":1133136000000},"content-version":"vor","delay-in-days":7942,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis & Rheumatism"],"published-print":{"date-parts":[[1984,3]]},"abstract":"Abstract<\/jats:title>Results of the solid phase F(ab')2<\/jats:sub> anti\u2010C3 radio\u2010immunoassay for immune complexes were abnormal in sera from some patients with juvenile arthritis and in many patients with other forms of chronic inflammation. However, C3 failed to block positive reactions of sera from patients even though it did block the reaction when aggregated IgG was added to normal sera. Moreover, positive sera reacted with nonspecific F(ab')2<\/jats:sub> fragments from several species. High correlation coefficients were obtained between abnormal results in the anti\u2010C3 assay with juvenile arthritis sera and reactions with goat, mouse, and rabbit F(ab')2<\/jats:sub>, but less so with human F(ab')2<\/jats:sub>. Sera from patients with systemic lupus erythematosus or cystic fibrosis had similar reactions but different species specificities. After sucrose density fractionation, the reactivity with nonspecific anti\u2010F(ab')2<\/jats:sub> was only in the IgG\u2010containing fractions, but anti\u2010C3 activity was in both IgG\u2010containing fractions and the fractions between IgG and IgM. We conclude that anti\u2010F(ab')2<\/jats:sub> antibodies are common in children with inflammation and may interfere with interpretation of assays using F(ab')2<\/jats:sub> anti\u2010C3 for detection of complexes.<\/jats:p>","DOI":"10.1002\/art.1780270313","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T20:51:59Z","timestamp":1171486319000},"page":"330-336","source":"Crossref","is-referenced-by-count":16,"title":["Anti\u2010f(ab\u2032)2<\/sub> antibodies that interfere with interpretation of the anti\u2010c3 assay for immune complexes in children with rheumatic diseases"],"prefix":"10.1002","volume":"27","author":[{"given":"Lynne C.","family":"Olds","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John J. III","family":"Miller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,28]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780200807"},{"key":"e_1_2_1_3_2","first-page":"665","article-title":"C1q binding in serum in juvenile rheumatoid arthritis","volume":"7","author":"Miller JJ","year":"1980","journal-title":"J Rheumatol"},{"key":"e_1_2_1_4_2","first-page":"395","article-title":"Immune complexes in juvenile rheumatoid arthritis: a comparison of four methods","volume":"9","author":"Moore TL","year":"1982","journal-title":"J Rheumatol"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.28.4.424"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-198010000-00010"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780200214"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780220907"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1159\/000460705","article-title":"Anti\u2010gammaglobulin factors in human sera revealed by enzymatic splitting of anti\u2010Rh antibodies","volume":"8","author":"Osterland CK","year":"1963","journal-title":"Vox Sang"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1159\/000232854"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(82)90167-2"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780251105"},{"key":"e_1_2_1_13_2","first-page":"S195","article-title":"Current proposed revision of JRA criteria","volume":"20","author":"Brewer EJ","year":"1977","journal-title":"Arthritis Rheum (suppl)"},{"key":"e_1_2_1_14_2","doi-asserted-by":"crossref","first-page":"763","DOI":"10.4049\/jimmunol.125.2.763","article-title":"Detection and partial characterization of circulating immune complexes with solid\u2010phase anti\u2010C3","volume":"125","author":"Pereira AB","year":"1980","journal-title":"J Immunol"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj0890114"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.72.6.1989"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(76)90148-4"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780220510"},{"key":"e_1_2_1_19_2","doi-asserted-by":"crossref","first-page":"232","DOI":"10.4049\/jimmunol.116.1.232","article-title":"Detection of immune complexes in unheated sera by a modified 125I\u2010Clq binding test","volume":"116","author":"Zubler RH","year":"1976","journal-title":"J Immunol"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(81)80453-2"},{"key":"e_1_2_1_21_2","doi-asserted-by":"crossref","first-page":"438","DOI":"10.4049\/jimmunol.97.3.438","article-title":"Further studies on the antiglobulin factors in human serum to the pepsin digested fragment of the Ri anti\u2010Rh antibody","volume":"97","author":"Waller M","year":"1966","journal-title":"J Immunol"},{"key":"e_1_2_1_22_2","first-page":"451","article-title":"The influence of infection on titres of antiglobulin antibodies","volume":"8","author":"Waller M","year":"1971","journal-title":"Clin Exp Immunol"}],"container-title":["Arthritis & Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.1780270313","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.1780270313","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T05:50:56Z","timestamp":1700718656000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.1780270313"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,3]]},"references-count":21,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1984,3]]}},"alternative-id":["10.1002\/art.1780270313"],"URL":"https:\/\/doi.org\/10.1002\/art.1780270313","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[1984,3]]}}}