{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:45:35Z","timestamp":1759333535687},"reference-count":18,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,11,28]],"date-time":"2005-11-28T00:00:00Z","timestamp":1133136000000},"content-version":"vor","delay-in-days":7605,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis & Rheumatism"],"published-print":{"date-parts":[[1985,2]]},"abstract":"Abstract<\/jats:title>The Crithidia luciliae<\/jats:italic> immunofluorescence (CLIF) assay is widely used to test for native DNA (nDNA) antibodies in the diagnosis and management of systemic lupus erythematosus. However, sera from patients with drug\u2010induced lupus erythematosus or rheumatoid arthritis, which should not contain nDNA antibodies, occasionally react with the CL kinetoplast. We examined 36 sera from patients with systemic lupus erythematosus, rheumatoid arthritis, Sj\u00f6gren's syndrome, and drug\u2010induced lupus erythematosus, who had positive CLIF tests. All 36 sera were also antinuclear antibody\u2010positive with homogeneous and\/or peripheral staining patterns on mouse kidney substrates. After hydrochloric acid extraction of the CL smears to remove histone and other nuclear protein antiges, 14 of the 36 sera no longer produced a positive result on the CLIF test. Ten of these 14 sera again gave a positive CLIF result after the hydrochloric acid\u2010extracted Crithidia<\/jats:italic> substrate had been reconstituted with purified histone. These studies demonstrated that kinetoplast binding was due to antihistone antibodies in at least 10 of 36 initially CLIF\u2010positive sera. Antihistone antibodies were then purified with a histone\u2010affinity column, and these purified antibodies were reactive with CL kinetoplasts. Thus, the CLIF test is not specific for nDNA antibodies. Additional studies using CL from different days of culture indicated that histone antigen expression in the CL kinetoplast was a function of the life cycle of this organism and is most readily detected 2 days after initiation of culture.<\/jats:p>","DOI":"10.1002\/art.1780280210","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T21:04:34Z","timestamp":1171487074000},"page":"163-168","source":"Crossref","is-referenced-by-count":21,"title":["The binding of antihistone antibodies to crithidia luciliae<\/i> kinetoplasts is growth cycle\u2010dependent"],"prefix":"10.1002","volume":"28","author":[{"given":"Jau\u2010Shyong","family":"Deng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard D.","family":"Sontheimer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary F.","family":"Lipscomb","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James N.","family":"Gilliam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,28]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1975.tb29197.x"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(76)90103-3"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.3109\/03009747609099909"},{"key":"e_1_2_1_5_2","first-page":"480","article-title":"The Crithidia luciliae kinetoplast immunofluorescent test in systemic lupus erythematosus","volume":"25","author":"Slater NG","year":"1976","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780200308"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1093\/ajcp\/69.6.599","article-title":"Antibodies to double\u2010stranded DNA: a comparison of the indirect immunofluorescent test using Crithidia luciliae and the DNA\u2010binding assay","volume":"69","author":"Deegan MJ","year":"1978","journal-title":"Am J Clin Pathol"},{"key":"e_1_2_1_8_2","first-page":"550","article-title":"An immunofluorescence assay for double\u2010stranded DNA antibodies using Crithidia luciliae kinetoplast as a double\u2010stranded DNA substrate","volume":"91","author":"Sontheimer RD","year":"1978","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-89-2-186"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1093\/ajcp\/72.5.829","article-title":"Clinical usefulness of the Crithidia luciliae test for antibodies to native DNA","volume":"72","author":"Whiteside TL","year":"1979","journal-title":"Am J Clin Pathol"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.34.9.1032"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0047-0740(82)90038-9"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(84)70197-6"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/82.4.448"},{"key":"e_1_2_1_15_2","volume-title":"Primer on the Rheumatic Diseases","author":"Rodnan GP","year":"1983"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1976.tb03030.x"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI109161"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780240813"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(82)90166-0"}],"container-title":["Arthritis & Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.1780280210","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.1780280210","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T21:46:03Z","timestamp":1700689563000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.1780280210"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,2]]},"references-count":18,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1985,2]]}},"alternative-id":["10.1002\/art.1780280210"],"URL":"https:\/\/doi.org\/10.1002\/art.1780280210","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,2]]}}}