{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T10:36:11Z","timestamp":1773657371660,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,11,28]],"date-time":"2005-11-28T00:00:00Z","timestamp":1133136000000},"content-version":"vor","delay-in-days":7605,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1985,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dermal fibroblast cultures from patients with progressive systemic sclerosis (PSS) synthesize up to 5 times more glycosaminoglycan (GAG) than normal cultures. In an in vitro model of fibroblast\u2010lymphocyte interactions, we show that the supernatants of activated mononuclear cells (MNC) modulate GAG synthesis, as measured by the incorporation of <jats:sup>3<\/jats:sup>H\u2010glucosamine into GAG following incubation of the confluent fibroblast monolayers with active supernatant preparations. GAG accumulation was selectively increased up to 18 times in normal dermal fibroblast cultures. Cell viability was not affected, and <jats:sup>3<\/jats:sup>H\u2010thymidine uptake and cell numbers were depressed in cultures treated with the supernatants. In contrast to normal dermal fibroblast cultures, PSS fibroblasts responded to MNC supernatants by only a 1\u20132\u2010fold increase in GAG. Supernatants of concanavalin A\u2010activated PSS MNC had higher stimulatory activity than those of normal MNC. Supernatants made with MNC that had been depleted of monocytes on Sephadex G\u201010 columns were only minimally stimulatory. The GAG\u2010stimulatory supernatants modulated the synthesis, but not the degradation of GAG. Gel filtration on a calibrated Sephadex G\u2010100 column indicated the presence of stimulatory activity in both the 50,000 and 15,000 molecular weight fractions. These activities were trypsin\u2010sensitive, but had different susceptibilities to heat. The active column fractions also contained interleukin\u20101 activity, as shown in an assay measuring proliferation of mouse thymocytes. Like our factors, interleukin\u20101 preparations increased GAG in normal and PSS dermal fibroblasts. Products of activated MNC may modulate normal and pathologic processes in human skin.<\/jats:p>","DOI":"10.1002\/art.1780280214","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T21:04:34Z","timestamp":1171487074000},"page":"188-197","source":"Crossref","is-referenced-by-count":46,"title":["Soluble mediators from mononuclear cells increase the synthesis of glycosaminoglycan by dermal fibroblast cultures derived from normal subjects and progressive systemic sclerosis patients"],"prefix":"10.1002","volume":"28","author":[{"given":"Theresa L.","family":"Whiteside","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer G.","family":"Worrall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert K.","family":"Prince","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert B.","family":"Buckingham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerald P.","family":"Rodnan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,28]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"5","article-title":"Increased collagen accumulation in dermal fibroblast cultures from patients with progressive systemic sclerosis (scleroderma)","volume":"92","author":"Buckingham RB","year":"1978","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.135.6.1351"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780200318"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"401","DOI":"10.2340\/0001555551401406","article-title":"Connective tissue in scleroderma: a biochemical study on the correlation of fractionated glycosaminoglycans and collagen in human skin","volume":"51","author":"Uitto J","year":"1971","journal-title":"Acta Derm Venereol"},{"key":"e_1_2_1_6_2","first-page":"659","article-title":"Progressive systemic sclerosis (PSS, scleroderma) dermal fibroblasts synthesize increased amounts of glycosaminoglycan","volume":"101","author":"Buckingham RB","year":"1983","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_7_2","first-page":"S61","article-title":"Quantitation and subtyping of lymphocytes in skin of patients with progressive systemic sclerosis (abstract)","volume":"26","author":"Roumm A","year":"1983","journal-title":"Arthritis Rheum (suppl)"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI108455"},{"key":"e_1_2_1_9_2","first-page":"S14","article-title":"The effect of lymphokines from normal and PSS lymphocytes on connective tissue synthesis in vitro (abstract)","volume":"25","author":"Whiteside TL","year":"1982","journal-title":"Arthritis Rheum (suppl)"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.188134"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI109698"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780240610"},{"key":"e_1_2_1_13_2","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/S0307-742X(21)00050-3","article-title":"Classification and nomenclature of progressive systemic sclerosis (scleroderma)","volume":"5","author":"Rodnan GP","year":"1979","journal-title":"Clin Rheum Dis"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780230209"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780110507"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(80)90113-1"},{"key":"e_1_2_1_17_2","first-page":"557","article-title":"Use of avidin\u2010biotin\u2010peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures","volume":"29","author":"Hsu S","year":"1981","journal-title":"J Histochem Cytochem"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(81)90191-5"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.6.2844"},{"key":"e_1_2_1_20_2","doi-asserted-by":"crossref","first-page":"1497","DOI":"10.4049\/jimmunol.120.5.1497","article-title":"Characterization of lymphocyte\u2010activating factor (LAF) produced by the macrophage cell line P388D1. 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