{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:28:44Z","timestamp":1791300524487,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2005,11,28]],"date-time":"2005-11-28T00:00:00Z","timestamp":1133136000000},"content-version":"vor","delay-in-days":7455,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1985,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Antibodies to Mi, an antigen in calf thymus extract, have been demonstrated by complement fixation inhibition in polymyositis (PM) and dermatomyositis (DM) sera but not in the sera of individuals without myositis. The original Mi reference serum defined 2 precipitating antibodies, using immunodiffusion (ID). Anti\u2010Mi\u20101 was not active in complement fixation. We have now studied in further detail anti\u2010Mi\u20102, which appears to be the antibody in Mi serum that fixes complement. Mi\u20102 antigen was purified by immunoaffinity chromotography. An enzyme\u2010linked immunosorbent assay (ELISA) to measure Mi\u20102 antibody, using this antigen, was used to test the sera of 139 myositis patients: 52 had DM and 87 had PM. Control sera from 35 normal subjects and 93 patients with other connective tissue diseases were also tested. Only 13 sera were considered definitely positive for anti\u2010Mi\u20102. All were from patients who had myositis, 11 of whom had DM. Only DM sera had anti\u2010Mi\u20102 by ID, and all sera with anti\u2010Mi\u20102 by ID were positive by ELISA. A number of other sera, including many from patients with other connective tissue diseases and 2 from normal subjects (all without precipitating antibodies) had lower elevations which were of uncertain significance. Detection of anti\u2010Mi\u20102 by ID as well as by ELISA was significantly more frequent in DM than in PM. Anti\u2010Mi\u20102 appears to be closely linked to DM, and is the first specific serologic marker for this form of myositis.<\/jats:p>","DOI":"10.1002\/art.1780280711","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T20:55:40Z","timestamp":1171486540000},"page":"796-803","source":"Crossref","is-referenced-by-count":226,"title":["The association between Mi\u20102 antibodies and dermatomyositis"],"prefix":"10.1002","volume":"28","author":[{"given":"Ira N.","family":"Targoff","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morris","family":"Reichlin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,11,28]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780271011"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00915286"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780230802"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI110295"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(76)90145-8"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(80)90109-1"},{"key":"e_1_2_1_8_2","first-page":"76","article-title":"Antibodies to Mi\u20101 in SLE: relationship to other precipitins and reaction with bovine immunoglobulin","volume":"53","author":"Targoff IN","year":"1983","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197502132920706"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.4049\/jimmunol.107.5.1281","article-title":"Characterization of a soluble nuclear ribonucleoprotein antigen reactive with SLE sera","volume":"107","author":"Mattioli M","year":"1971","journal-title":"J Immunol"},{"key":"e_1_2_1_11_2","first-page":"43","volume-title":"The isolation and characterization of immunoglobulins, antibodies, and their constituent polypeptide chains, Immunological Methods","author":"Jaton JC","year":"1979"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj0920313"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_1_15_2","first-page":"1298","volume-title":"Dermatomyositis, Dermatology in General Medicine","author":"Mills JA","year":"1979"},{"key":"e_1_2_1_16_2","first-page":"925","article-title":"The Jo\u20101 antibody system in myositis: relationship to clinical features and HLA","volume":"8","author":"Arnett FC","year":"1981","journal-title":"J Rheumatol"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/304177a0"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1984.tb08022.x"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.160.2.420"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/11.3.853"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(78)90053-6"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.1780280711","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.1780280711","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T00:13:45Z","timestamp":1700698425000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.1780280711"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,7]]},"references-count":20,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1985,7]]}},"alternative-id":["10.1002\/art.1780280711"],"URL":"https:\/\/doi.org\/10.1002\/art.1780280711","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,7]]}}}