{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T08:27:49Z","timestamp":1765700869515},"reference-count":16,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,11,29]],"date-time":"2005-11-29T00:00:00Z","timestamp":1133222400000},"content-version":"vor","delay-in-days":7272,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1986,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A distinctive type of speckled antinuclear antibody staining pattern was identified by indirect immunofluorescence on mouse kidney substrate in 4.8% of 5,976 specimens analyzed for antinuclear antibodies. This pattern, termed variable large speckles (VLS), consisted of 3\u201310 nuclear speckles ranging in size from approximately 0.2\u20132.0\u03bc. The pattern could be differentiated from other indirect immunofluorescence patterns related to specific antibodies. The predominant immunoglobulin isotype demonstrating the VLS pattern was IgM in 27 of 28 sera examined and IgG in 1 serum. VLS sera had substantial IgM antibodies to histone demonstrated by enzyme immunoassay, and further analysis of representative sera showed predominant antibody activity to histone class 3 (H\u20103). Adsorption with histone H\u20103 resulted in decrease or removal of antibody producing the VLS pattern. Available information showed that most patients with IgM antibodies of the VLS pattern had undifferentiated connective tissue disease symptoms. They were characterized by a heterogeneity of chronic symptoms including arthralgias, myalgias, inflammatory polyarthritis, myositis, sicca symptoms, and pleurisy associated with elevation of the erythrocyte sedimentation rate. It remains to be determined whether the IgM anti\u2010histone H\u20103 profile of these patients is a transient or long\u2010standing serologic characteristic.<\/jats:p>","DOI":"10.1002\/art.1780290106","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T21:20:07Z","timestamp":1171488007000},"page":"39-46","source":"Crossref","is-referenced-by-count":23,"title":["IgM anti\u2010histone H\u20103 antibody associated with undifferentiated rheumatic disease syndromes"],"prefix":"10.1002","volume":"29","author":[{"given":"Daniel P.","family":"Molden","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary L.","family":"Klipple","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carol L.","family":"Peebles","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert L.","family":"Rubin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert M.","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eng M.","family":"Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,29]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(83)80283-4"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.3.1627"},{"key":"e_1_2_1_4_2","first-page":"77","article-title":"Separation of leukocytes from blood and bone marrow","volume":"21","author":"Borjum A","year":"1968","journal-title":"Scand J Lab Invest (suppl)"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"442","DOI":"10.4049\/jimmunol.95.3.442","article-title":"The solubility and other physiochemical properties of human gamma globulin labeled with fluorescein isothiocyanate","volume":"95","author":"Frommhagen L","year":"1965","journal-title":"J Immunol"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(72)90013-X"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI108007"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60836-6"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780250814"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(83)80009-X"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"269","DOI":"10.4049\/jimmunol.131.1.269","article-title":"In vivo and in vitro production of anti\u2010histone antibodies in NZB\/NZW mice","volume":"131","author":"Gioud M","year":"1983","journal-title":"J Immunol"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1976.tb03030.x"},{"key":"e_1_2_1_13_2","doi-asserted-by":"crossref","first-page":"95","DOI":"10.4049\/jimmunol.97.1.95","article-title":"On the nature of complement\u2010fixing antibodies to Mycoplasma pneumonia","volume":"97","author":"Schmidt NJ","year":"1966","journal-title":"J Immunol"},{"key":"e_1_2_1_14_2","first-page":"113","article-title":"A modification of the latex\u2010fixation test for the study of rheumatoid arthritis","volume":"50","author":"Rheins MS","year":"1957","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_15_2","first-page":"S44","article-title":"Specificity of anti\u2010histone antibodies and relation to clinical symptoms in drug\u2010induced lupus (abstract)","volume":"27","author":"Rubin RL","year":"1984","journal-title":"Arthritis Rheum (suppl)"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780270114"},{"key":"e_1_2_1_17_2","first-page":"76","article-title":"Cold\u2010reacting antinuclear factor in sera from patients with IgA nephropathy","volume":"94","author":"Nomoto Y","year":"1979","journal-title":"J Lab Clin Med"}],"container-title":["Arthritis &amp; 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