{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T16:25:39Z","timestamp":1759422339611},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,11,29]],"date-time":"2005-11-29T00:00:00Z","timestamp":1133222400000},"content-version":"vor","delay-in-days":6817,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1987,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Combined DEAE\u2010Sephacel and hydroxyapatite chromatography resulted in a separation of free Sm antigen from the Sm\/RNP complex in rabbit thymus extracts. In Western blots, the free Sm preparation contained an immunoreactive 14\u2010kd (D) protein, whereas the Sm\/RNP complex contained, in addition to the 14\u2010kd protein, a 68\u2010kd\u2014reactive protein and its putative degradation fragments. Ethidium bromide staining of these preparations separated by agarose gel electrophoresis showed that the Sm\/RNP preparation contained RNA, but the free Sm preparation did not. These preparations were used as antigens in an enzyme\u2010linked immunosorbent assay (ELISA). Sera characterized as anti\u2010Sm only, anti\u2010Sm\/RNP, and anti\u2010RNP only were assayed. A quotient (Q) was derived from the ELISA optical density obtained with Sm\/RNP as antigen divided by the optical density obtained with free Sm as antigen. Q values &lt;4.0 characterized sera with anti\u2010Sm only, values of 4.0\u201312.0 were observed in sera with anti\u2010Sm plus anti\u2010RNP, and values &gt;12.0 in sera with anti\u2010RNP only. Sera with antibodies to other nuclear antigens were not reactive in this system. It has traditionally been difficult to identify sera with anti\u2010RNP when this is present simultaneously with, and in lower concentration than, anti\u2010Sm. With this method, such sera can be identified by their Q value, which falls in an intermediate region between a lower Q for anti\u2010Sm and a higher Q for anti\u2010RNP.<\/jats:p>","DOI":"10.1002\/art.1780300405","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T21:39:58Z","timestamp":1171489198000},"page":"389-396","source":"Crossref","is-referenced-by-count":16,"title":["An immunoassay differentiating sera with antibodies to sm alone, antibodies to sm\/RNP complex, and antibodies to RNP alone"],"prefix":"10.1002","volume":"30","author":[{"given":"Gregory J.","family":"Tsay","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward K. L.","family":"Chan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carol L.","family":"Peebles","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. Michael","family":"Pollard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eng M.","family":"Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,29]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60836-6"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-83-4-464"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(72)90064-2"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(72)90013-X"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"1318","DOI":"10.4049\/jimmunol.110.5.1318","article-title":"Physical association of two nuclear antigens and mutual occurrence of their antibodies: the relationship of the Sm and RNP protein (Mo) systems in SLE sera","volume":"110","author":"Mattioli M","year":"1973","journal-title":"J Immunol"},{"key":"e_1_2_1_7_2","first-page":"8","article-title":"Separation of two molecular species of the Sm antigen by affinity chromatography with murine monoclonal and human anti\u2010nuclear autoantibodies","volume":"51","author":"Buchanan RRC","year":"1983","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.11.5495"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.10.3185"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"2604","DOI":"10.1016\/S0021-9258(18)32969-7","article-title":"Isolation of small nuclear ribonucleoproteins containing U1, U2, U4, U5, and U6 RNA's","volume":"258","author":"Hinterberger M","year":"1983","journal-title":"J Biol Chem"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.156.5.1475"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780190309"},{"key":"e_1_2_1_13_2","first-page":"586","article-title":"Purification of the Sm nuclear autoantigen: detection and clinical significance of IgM antibody","volume":"60","author":"Pollard KM","year":"1985","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(83)80009-X"},{"key":"e_1_2_1_16_2","first-page":"175","volume-title":"Anti\u2010DNA antibodies, Methods of Enzymatic Analysis","author":"Rubin RL","year":"1986"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_1_18_2","first-page":"149","volume-title":"Molecular Cloning, A Laboratory Manual","author":"Maniatis T","year":"1982"},{"key":"e_1_2_1_19_2","first-page":"5907","article-title":"The structure of mammalian small nuclear ribonucleoproteins","volume":"259","author":"Pettersson I","year":"1984","journal-title":"J Biol Chem"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI111734"},{"key":"e_1_2_1_21_2","doi-asserted-by":"crossref","first-page":"2751","DOI":"10.4049\/jimmunol.128.6.2751","article-title":"Assays for the Sm and RNP autoantigens: the requirement for RNA and influence of the tissue source","volume":"128","author":"White PJ","year":"1982","journal-title":"J Immunol"},{"key":"e_1_2_1_22_2","doi-asserted-by":"crossref","first-page":"347","DOI":"10.4049\/jimmunol.131.1.347","article-title":"Isolation of intact Sm\/RNP antigens from rabbit thymus","volume":"131","author":"Billings PB","year":"1983","journal-title":"J Immunol"},{"key":"e_1_2_1_23_2","first-page":"133","volume-title":"Non histone protein antigens in human rheumatic and connective tissue disease, Chromosomal Nonhistone Proteins","author":"MacGillivray AJ","year":"1982"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.1780300405","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.1780300405","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T19:38:14Z","timestamp":1696189094000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.1780300405"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,4]]},"references-count":22,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1987,4]]}},"alternative-id":["10.1002\/art.1780300405"],"URL":"https:\/\/doi.org\/10.1002\/art.1780300405","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,4]]}}}