{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T04:46:20Z","timestamp":1696221980955},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,11,29]],"date-time":"2005-11-29T00:00:00Z","timestamp":1133222400000},"content-version":"vor","delay-in-days":6817,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1987,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have previously presented evidence that an intra\u2010erythrocyte arylesterase is primarily responsible for the deacetylation of aspirin to salicylate. We now report studies on extracellular control factors. The fractional acetylsaticylic acid (ASA) hydrolysis at the completion of 20\u2010minute incubations in suspensions of washed red blood cells (RBC) with human serum albumin (HSA) decreased sharply, from 0.6 at 0 gm\/dl HSA to 0.2 at 5.0 gm\/dl HSA. In RBC suspensions with oleic, linoleic, and lauric acid, ASA hydrolysis increased as fatty acid:HSA ratios increased. The addition of salicylate (SA) also resulted in an increase in the fraction hydrolyzed. In a separate, more direct, ultracentrifugation assay, unbound ASA levels were similarly affected by HSA and the other ligands. When SA and oleic acid were incubated with ASA in RBC, additive competition for binding sites was apparent at relatively low levels of the fatty acid. In the presence of increasing concentrations of sodium SA, 5\u2010amino SA, and 4\u2010amino SA, ASA hydrolysis in RBC suspensions was increased by an average of 137%, 75%, and 54%, respectively. The most reasonable explanation for these findings is that the intact ASA molecule binds to albumin and that the binding of competing ligands can modulate the levels of free ASA in the circulation. This, in turn, affects the amount of drug available as substrate for penetration into the RBC and reaction with the intracellular hydrolase which catalyzes ASA deacetylation. Such factors controlling drug availability, if validated in vivo, could be important with regard to the antiinflammatory and anti\u2010platelet properties of aspirin.<\/jats:p>","DOI":"10.1002\/art.1780300408","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T21:39:58Z","timestamp":1171489198000},"page":"412-418","source":"Crossref","is-referenced-by-count":0,"title":["The extracellular control of intracellular aspirin hydrolysis"],"prefix":"10.1002","volume":"30","author":[{"given":"Patrick B.","family":"Costello","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Floyd A.","family":"Green","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,29]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780250509"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780260414"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780270409"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(81)90151-9"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1201\/b14095","volume-title":"Pharmacokinetics","author":"Gibaldi M","year":"1982"},{"key":"e_1_2_1_7_2","first-page":"161","article-title":"The binding of salicylic acid and related substances to purified proteins","volume":"133","author":"Davison C","year":"1961","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-9120(73)80018-9"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-7158.1980.tb12991.x"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.72.8.3073"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI108941"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1016\/S0021-9258(19)45689-5","article-title":"Erythrocyte membrane lipids and Rh antigen activity","volume":"247","author":"Green FA","year":"1972","journal-title":"J Biol Chem"},{"key":"e_1_2_1_13_2","first-page":"175","volume-title":"Determination of serum albumin, Standard Methods of Clinical Chemistry","author":"Doumas BT","year":"1972"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)81445-X"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600670540"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600570807"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600610312"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198411083111902"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja01548a024"},{"key":"e_1_2_1_20_2","first-page":"261","article-title":"Effect of free fatty acids on binding of drugs by bovine serum albumin, by human serum albumin and by rabbit serum","volume":"176","author":"Rudman D","year":"1971","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1973.tb20486.x"},{"key":"e_1_2_1_22_2","first-page":"159","article-title":"Molecular features of organic anion permeability in ox red blood cell","volume":"246","author":"Aubert L","year":"1975","journal-title":"J Physiol (Paris)"},{"key":"e_1_2_1_23_2","first-page":"882","article-title":"Patterns of in vitro aspirin hydrolysis rates in rheumatoid arthritis and systemic lupus erythematosus","volume":"13","author":"Costello PB","year":"1986","journal-title":"J Rheumatol"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197807132990201"},{"key":"e_1_2_1_25_2","first-page":"90","article-title":"Controlled clinical trial of aspirin in cerebral ischemia","volume":"62","author":"Fields WS","year":"1980","journal-title":"Circulation (suppl)"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198308183090703"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(83)90527-2"},{"key":"e_1_2_1_28_2","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1093\/oxfordjournals.bmb.a071836","article-title":"Clinical aspects of the arachiodonic acid\u2010thromboxane pathway","volume":"39","author":"Mitchell JRA","year":"1983","journal-title":"Br Med Bull"}],"container-title":["Arthritis &amp; 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