{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T15:30:56Z","timestamp":1770478256038,"version":"3.49.0"},"reference-count":23,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2005,11,29]],"date-time":"2005-11-29T00:00:00Z","timestamp":1133222400000},"content-version":"vor","delay-in-days":6268,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1988,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Clones of dermal fibroblasts from the skin of 4 normal subjects and 5 patients with progressive systemic sclerosis (PSS; scleroderma) were established, and their synthetic and proliferative characteristics were compared. A limiting\u2010dilution assay was used to determine frequencies of cloning in the microcultures of dermal fibroblasts plated. The clones derived from single cells were expanded in vitro and examined (in passages C\u2013H) for growth and synthesis of glycosaminoglycan (GAG) and collagenase\u2010sensitive protein (CSP). The clonogenicity of PSS fibroblasts was not significantly different from that of normal fibroblasts. Normal fibroblast clones were characterized by low levels of GAG and CSP synthesis, and there was a correlation between the GAG and CSP phenotypes. In contrast, clones of PSS fibroblasts were often, but not always, high producers of GAG and CSP, but there was no correlation between the levels of GAG and CSP synthesis. It appears that scleroderma skin is composed of fibroblast clones that are unable to regulate the synthesis of connective tissue components and often synthesize large amounts of connective tissue macromolecules.<\/jats:p>","DOI":"10.1002\/art.1780311002","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T21:55:49Z","timestamp":1171490149000},"page":"1221-1229","source":"Crossref","is-referenced-by-count":28,"title":["Heterogeneous synthetic phenotype of cloned scleroderma fibroblasts may be due to aberrant regulation in the synthesis of connective tissues"],"prefix":"10.1002","volume":"31","author":[{"given":"Theresa L.","family":"Whiteside","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina","family":"Ferrarini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Hebda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert B.","family":"Buckingham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,29]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"Progressive systemic sclerosis (scleroderma), Arthritis and Allied Conditions","author":"Rodnan GP","year":"1979"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"401","DOI":"10.2340\/0001555551401406","article-title":"Connective tissue in scleroderma: a biochemical study on the correlation of fractionated glycosaminoglycans and collagen in human skin","volume":"51","author":"Uitto J","year":"1971","journal-title":"Acta Derm Venereol (Stockh)"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12538919"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780220205"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107827"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780250212"},{"key":"e_1_2_1_8_2","first-page":"659","article-title":"Progressive systemic sclerosis (PSS, scleroderma) dermal fibroblasts synthesize increased amounts of glycosaminoglycan","volume":"101","author":"Buckingham RB","year":"1983","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_9_2","first-page":"5","article-title":"Increased collagen accumulation in dermal fibroblast cultures from patients with progressive systemic sclerosis (scleroderma)","volume":"92","author":"Buckingham RB","year":"1978","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780200323"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.135.6.1351"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240280204"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780300407"},{"key":"e_1_2_1_14_2","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/S0307-742X(21)00050-3","article-title":"Classification and nomenclature of progressive systemic sclerosis (scleroderma)","volume":"5","author":"Rodnan GP","year":"1979","journal-title":"Clin Rheum Dis"},{"key":"e_1_2_1_15_2","doi-asserted-by":"crossref","first-page":"1614","DOI":"10.4049\/jimmunol.126.4.1614","article-title":"Limiting dilution assays for the determinations of immunocompetent cell frequencies. 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Data analysis","volume":"126","author":"Taswell C","year":"1981","journal-title":"J Immunol"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780280214"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00782a009"},{"key":"e_1_2_1_18_2","first-page":"355","article-title":"Products of activated mononuclear cells modulate accumulation of collagen by normal dermal and scleroderma fibroblasts in culture","volume":"104","author":"Whiteside TL","year":"1984","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780290108"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780270208"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0174-173X(81)80027-1"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.4.1286"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6691142"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI110873"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.1780311002","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.1780311002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T04:40:42Z","timestamp":1700714442000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.1780311002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,10]]},"references-count":23,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1988,10]]}},"alternative-id":["10.1002\/art.1780311002"],"URL":"https:\/\/doi.org\/10.1002\/art.1780311002","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,10]]}}}