{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T04:30:48Z","timestamp":1777437048357,"version":"3.51.4"},"reference-count":46,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,12,9]],"date-time":"2005-12-09T00:00:00Z","timestamp":1134086400000},"content-version":"vor","delay-in-days":5790,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1990,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mouse monoclonal antibody (MAb) 6B6.6 was raised against a cross\u2010reactive idiotope (CRI) present on the light chains of 2 human IgM paraproteins with rheumatoid factor (RF) activity. The MAb inhibited the IgG\u2010binding activity of these proteins, and thus appears to react with an epitope located at or near the RF\u2010binding site. Enzyme\u2010linked immunosorbent assay (ELISA) and Western immunoblotting studies indicate that the 6B6.6 CRI is associated with <jats:italic>k<\/jats:italic>IIIa sub\u2010subgroup light chains, is not related to the Wa, Po, and Bla RF cross\u2010idiotypic specificities, and is clearly distinct from the <jats:italic>k<\/jats:italic>IIIb\u2010associated CRI detected by MAb 17.109. Using an ELISA, we detected 6B6.6 CRI in 59% of 107 sera and 48% of 50 synovial fluids from patients with seropositive rheumatoid arthritis (RA). However, the quantities of CRI\u2010positive RF were small, and the amount of CRI\u2010positive RF did not correlate with the amount of IgM\u2010RF. The 6B6.6 CRI was shown to occur primarily in the IgM fraction of RA sera by both chromatographic studies and isotype\u2010specific ELISA, although small quantities appeared to be associated with IgA and IgG in some sera. The presence of 6B6.6 CRI on both monoclonal and polyclonal RF is consistent with the view that both are derived, at least in part, from a common gene pool. However, its occurrence in relatively low levels suggests that the number of germline genes encoding for RF is large or that extensive mutation occurs in the course of RF expression in RA.<\/jats:p>","DOI":"10.1002\/art.1780330206","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T23:03:53Z","timestamp":1171494233000},"page":"187-198","source":"Crossref","is-referenced-by-count":26,"title":["Monoclonal antibody 6b6.6 defines a cross\u2010reactive kappa light chain idiotope on human monoclonal and polyclonal rheumatoid factors"],"prefix":"10.1002","volume":"33","author":[{"given":"Ralph E.","family":"Schrohenloher","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary Ann","family":"Accavitti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ajit S.","family":"Bhown","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William J.","family":"Koopman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,9]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"Rheumatoid Arthritis","author":"Koopman WJ","year":"1985"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0019-2791(66)90276-X"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"295","DOI":"10.4049\/jimmunol.119.1.295","article-title":"Radioimmunoassay of IgG and IgM rheumatoid factors reacting with human IgG.","volume":"119","author":"Carson DA","year":"1977","journal-title":"J Immunol"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.38.2.161"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"576","DOI":"10.4049\/jimmunol.129.2.576","article-title":"Immunoglobulin A and polymeric IgA rheumatoid factors in systemic sicca syndrome: partial characterization.","volume":"129","author":"Elkon KB","year":"1982","journal-title":"J Immunol"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(82)90306-4"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780241208"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112522"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"536","DOI":"10.4049\/jimmunol.137.2.536","article-title":"Polymeric IgA rheumatoid factor in idiopathic IgA mesangial nephropathy (Berger's disease).","volume":"137","author":"Sinico RA","year":"1986","journal-title":"J Immunol"},{"key":"e_1_2_1_11_2","first-page":"177","article-title":"IgG antigens of the C\u03b32 and C\u03b33 homology regions interacting with rheumatoid factors.","volume":"12","author":"Natvig JB","year":"1972","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-116-29313"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1976.tb00309.x"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780230503"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.137.2.331"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1979.tb02732.x"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.151.6.1514"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(83)90116-5"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00915373"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1986.tb01955.x"},{"key":"e_1_2_1_21_2","doi-asserted-by":"crossref","first-page":"122","DOI":"10.4049\/jimmunol.137.1.122","article-title":"Expression of three cross\u2010reactive idiotypes on rheumatoid factor autoantibodies from patients with autoimmune diseases and seropositive adults.","volume":"137","author":"Fong S","year":"1986","journal-title":"J Immunol"},{"key":"e_1_2_1_22_2","doi-asserted-by":"crossref","first-page":"477","DOI":"10.4049\/jimmunol.136.2.477","article-title":"Expression of a cross\u2010reactive idiotype on rheumatoid factor in patients with Sj\u00f6gren's syndrome.","volume":"136","author":"Fox RI","year":"1986","journal-title":"J Immunol"},{"key":"e_1_2_1_23_2","first-page":"793A","article-title":"Expression of cross\u2010reactive idiotypes in a panel of monoclonal rheumatoid factors (abstract).","volume":"33","author":"Schrohenloher RE","year":"1985","journal-title":"Clin Res"},{"issue":"4","key":"e_1_2_1_24_2","first-page":"S28","article-title":"An idiotope common to rheumatoid factors from patients with rheumatoid arthritis identified by a monoclonal antibody (abstract).","volume":"29","author":"Schrohenloher RE","year":"1986","journal-title":"Arthritis Rheum"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI111097"},{"key":"e_1_2_1_26_2","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.4049\/jimmunol.134.3.1469","article-title":"Activity of rheumatoid factors of different molecular sizes: comparison of autologous monomeric and polymeric monoclonal IgA rheumatoid factors.","volume":"134","author":"Schrohenloher RE","year":"1985","journal-title":"J Immunol"},{"key":"e_1_2_1_27_2","first-page":"175","article-title":"1958 revision of diagnostic criteria for rheumatoid arthritis.","volume":"9","author":"Ropes MW","year":"1958","journal-title":"Bull Rheum Dis"},{"key":"e_1_2_1_28_2","unstructured":"FleischmanJB PainRH PorterRR: Reduction of \u03b3\u2010globulins. Arch Biochem Biophys (suppl 1):174\u2013180 1962"},{"key":"e_1_2_1_29_2","volume-title":"Biospecific affinity chromatography and related methods, The Proteins","author":"Porath J","year":"1975"},{"key":"e_1_2_1_30_2","doi-asserted-by":"crossref","first-page":"4073","DOI":"10.4049\/jimmunol.135.6.4073","article-title":"Sequence similarities and cross\u2010idiotypic specificity of L chains among human monoclonal IgMk with anti\u2010\u03b3\u2010globulin activity.","volume":"135","author":"Go\u00f1i F","year":"1985","journal-title":"J Immunol"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.142.1.106"},{"key":"e_1_2_1_32_2","doi-asserted-by":"crossref","first-page":"1548","DOI":"10.4049\/jimmunol.123.4.1548","article-title":"A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody\u2010secreting hybrid cell lines.","volume":"123","author":"Kearney JF","year":"1979","journal-title":"J Immunol"},{"key":"e_1_2_1_33_2","volume-title":"Fundamental Immunology","author":"Kearney JF","year":"1984"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780291003"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780310304"},{"key":"e_1_2_1_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(85)90535-4"},{"key":"e_1_2_1_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(86)90412-6"},{"key":"e_1_2_1_40_2","first-page":"261","article-title":"The amino acid sequence of the variable regions of the light chains from two idiotypically cross reactive IgM anti\u2010gamma globulins.","volume":"127","author":"Klapper DG","year":"1976","journal-title":"Ann Immunol (Paris)"},{"key":"e_1_2_1_41_2","volume-title":"Sequences of Proteins of Immunological Interest","author":"Kabat EA","year":"1987"},{"key":"e_1_2_1_42_2","doi-asserted-by":"crossref","first-page":"1322","DOI":"10.4049\/jimmunol.131.3.1322","article-title":"Preferential association of kIIIb light chains with monoclonal human IgMk autoantibodies.","volume":"131","author":"Ledford DK","year":"1983","journal-title":"J Immunol"},{"key":"e_1_2_1_43_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.139.1.128"},{"key":"e_1_2_1_44_2","doi-asserted-by":"crossref","first-page":"3411","DOI":"10.4049\/jimmunol.140.10.3411","article-title":"Incidence of three cross\u2010reactive idiotypes on human rheumatoid factor paraproteins.","volume":"140","author":"Crowley JJ","year":"1988","journal-title":"J Immunol"},{"key":"e_1_2_1_45_2","doi-asserted-by":"crossref","first-page":"2547","DOI":"10.4049\/jimmunol.142.7.2547","article-title":"Heterogeneity in the human Ig VH locus.","volume":"142","author":"Van Dijk KW","year":"1989","journal-title":"J Immunol"},{"key":"e_1_2_1_46_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.166.6.1900"},{"key":"e_1_2_1_47_2","doi-asserted-by":"crossref","first-page":"3281","DOI":"10.4049\/jimmunol.134.5.3281","article-title":"The majority of human monoclonal IgM rheumatoid factors express a \u201cprimary structure\u2010dependent\u201d cross\u2010reactive idiotype.","volume":"134","author":"Chen PP","year":"1985","journal-title":"J Immunol"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.1780330206","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.1780330206","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T22:20:56Z","timestamp":1700778056000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.1780330206"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,2]]},"references-count":46,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1990,2]]}},"alternative-id":["10.1002\/art.1780330206"],"URL":"https:\/\/doi.org\/10.1002\/art.1780330206","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,2]]}}}