{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T06:08:01Z","timestamp":1791094081331,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,12,9]],"date-time":"2005-12-09T00:00:00Z","timestamp":1134086400000},"content-version":"vor","delay-in-days":5731,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1990,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We report herein that cartilage proteolytic activity increased in bovine and rabbit articular cartilage after treatment with a purified staphylococcal culture medium or intraarticular infection with <jats:italic>Staphylococcus aureus.<\/jats:italic> Staphylococcal culture medium increased the release of gelatinolytic, collagenolytic, and caseinolytic activity into the medium of isolated chondrocytes or cartilage organ culture. The proteolytic activities were determined in assays using radiolabeled substrate and sodium dodecyl sulfate\u2010polyacrylamide gel electrophoresis. Staphylococcal culture medium was proteolytically inactive by both assay techniques. RNA synthesis of isolated chondrocytes was unaffected by staphylococcal culture medium, whereas overall protein synthesis was inhibited by 84%. An analysis of extracts of <jats:italic>Staphylococcus aureus<\/jats:italic>\u2010infected rabbit knee cartilage by substrate gels showed increased gelatinolytic and caseinolytic activity compared with extracts of uninfected knee cartilage. Our data suggest that the rapid loss of proteoglycan and persistent degradation of cartilage in staphylococcal septic arthritis is due to the production and activation of chondrocyte proteases.<\/jats:p>","DOI":"10.1002\/art.1780330411","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T23:29:27Z","timestamp":1171495767000},"page":"533-541","source":"Crossref","is-referenced-by-count":29,"title":["Septic arthritis"],"prefix":"10.1002","volume":"33","author":[{"suffix":"III","given":"Riley J.","family":"Williams","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Lane Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Schurman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,12,9]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780280217"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198503213121206"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/anr.1780320422"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.45.6.458"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.2106\/00004623-198769070-00015","article-title":"The effect of antibiotics on the destruction of cartilage in experimental infectious arthritis","volume":"69","author":"Smith RL","year":"1987","journal-title":"J Bone Joint Surg"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-197658030-00018"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1097\/00003086-198109000-00031","article-title":"The protective effect of continuous passive motion on living articular cartilage in acute septic arthritis","volume":"159","author":"Salter RB","year":"1981","journal-title":"Clin Orthop"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100010204"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780291111"},{"key":"e_1_2_1_11_2","first-page":"254","article-title":"Mechanisms of staphylococcal\u2010induced cartilage degradation (abstract)","volume":"11","author":"Smith RL","year":"1986","journal-title":"Trans Orthop Res Soc"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100060213"},{"key":"e_1_2_1_13_2","doi-asserted-by":"crossref","first-page":"6766","DOI":"10.1016\/S0021-9258(17)39794-6","article-title":"Properties of chick embryo chondrocyte grown in serum\u2010free medium","volume":"259","author":"Glaser JH","year":"1984","journal-title":"J Biol Chem"},{"key":"e_1_2_1_14_2","volume-title":"Selected Methods in Cellular Immunology","author":"Mishell BB","year":"1980"},{"key":"e_1_2_1_15_2","first-page":"861","article-title":"Mononuclear cell factors stimulate the concomitant secretion of distinct latent proteoglycan, gelatin and collagen degrading enzymes from human skin fibroblasts and synovial cells","volume":"10","author":"Golds EE","year":"1983","journal-title":"J Rheumatol"},{"key":"e_1_2_1_16_2","first-page":"20","article-title":"Human articular cartilage secretes characteristic metal dependent proteinases upon stimulation by mononuclear cell factor","volume":"13","author":"Campbell IK","year":"1986","journal-title":"J Rheumatol"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2090741"},{"key":"e_1_2_1_18_2","doi-asserted-by":"crossref","first-page":"12367","DOI":"10.1016\/S0021-9258(17)39034-8","article-title":"Stromelysin, a connective tissue\u2010degrading metalloendopeptidase secreted by stimulated rabbit synovial fibroblasts in parallel with collagenase","volume":"260","author":"Chin JR","year":"1985","journal-title":"J Biol Chem"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2410527"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(85)90056-X"},{"key":"e_1_2_1_21_2","doi-asserted-by":"crossref","first-page":"496","DOI":"10.4049\/jimmunol.138.2.496","article-title":"Human monocyte or recombinant interleukin l's are specific for the secretion of a metalloproteinase from chondrocytes","volume":"138","author":"Schnyder J","year":"1987","journal-title":"J Immunol"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.140.6.1482"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1510645"},{"key":"e_1_2_1_24_2","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1042\/bj1510665","article-title":"Rabbit collagenase: immunological identity of the enzymes released from cells and tissues in normal and pathological conditions","volume":"151","author":"Werb Z","year":"1975","journal-title":"Biochem J"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2140281"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2580115"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)69486-X"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2744(72)90249-5"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2744(81)90257-6"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(82)90340-5"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780250811"},{"key":"e_1_2_1_32_2","doi-asserted-by":"crossref","first-page":"3633","DOI":"10.1016\/S0021-9258(17)43141-3","article-title":"Two latent metalloproteinases of human articular cartilage that digest proteoglycan","volume":"259","author":"Woessner JF","year":"1984","journal-title":"J Biol Chem"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI108526"},{"key":"e_1_2_1_34_2","doi-asserted-by":"crossref","first-page":"2814","DOI":"10.1016\/S0021-9258(17)35860-X","article-title":"Secretion of metalloproteinases by stimulated capillary endothelial cells","volume":"261","author":"Herron GS","year":"1986","journal-title":"J Biol Chem"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0174-173X(84)80014-X"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)89580-1"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2030209"},{"key":"e_1_2_1_38_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1990807"},{"key":"e_1_2_1_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(83)90075-7"},{"key":"e_1_2_1_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-092150-8.50011-4"},{"key":"e_1_2_1_41_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197705052961801"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.1780330411","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.1780330411","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T22:43:52Z","timestamp":1700779432000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.1780330411"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,4]]},"references-count":40,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1990,4]]}},"alternative-id":["10.1002\/art.1780330411"],"URL":"https:\/\/doi.org\/10.1002\/art.1780330411","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,4]]}}}