{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,8]],"date-time":"2024-06-08T02:20:25Z","timestamp":1717813225531},"reference-count":29,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2010,8,17]],"date-time":"2010-08-17T00:00:00Z","timestamp":1282003200000},"content-version":"vor","delay-in-days":7321,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1990,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Synovial tissue samples from 6 patients with rheumatoid arthritis were cultured, and the IgG antibodies isolated from the synovial culture supernatants were used to immunize rabbits to make 6 antiidiotypic (anti\u2010Id) antibody preparations. After extensive adsorption, the rabbit anti\u2010Id were tested in a solid\u2010phase enzyme\u2010linked immunosorbent assay (ELISA). Each anti\u2010Id reacted predominantly with the immunizing synovial IgG and showed almost no reactivity with either pooled normal human serum IgG or with IgG from 50 normal donors. When identical amounts of matched rheumatoid arthritis serum IgG and synovial culture supernatant IgG were probed simultaneously with the corresponding rabbit anti\u2010Id in an ELISA, 3 of 6 pairs demonstrated an increased concentration of specific idiotypes in the synovial culture supernatant IgG. Furthermore, when these 6 matched samples were subsequently analyzed by isoelectric focusing, individual IgG antibodies in 5 of 6 synovial IgG samples revealed enhanced reactivity with the corresponding rabbit anti\u2010Id preparations, when compared with matched serum IgG. This increased synovial concentration of specific idiotypes detected by both the ELISA and isoelectric focusing was compatible with enhanced synovial tissue synthesis of the antibodies involved. These specific Id\/anti\u2010Id reactivities were not blocked by excess normal human Fc, Fab, or F(ab')<jats:sub>2<\/jats:sub> fragments, indicating a lack of association of the stimulating synovial antibodies with rheumatoid factors or antibodies against other IgG fragments (pepsin agglutinators).<\/jats:p>","DOI":"10.1002\/art.1780330821","type":"journal-article","created":{"date-parts":[[2010,8,18]],"date-time":"2010-08-18T07:02:03Z","timestamp":1282114923000},"page":"1196-1204","source":"Crossref","is-referenced-by-count":4,"title":["Synthesis of specific IgG idiotypes by rheumatoid synovium"],"prefix":"10.1002","volume":"33","author":[{"given":"Wayne L.","family":"Hoffman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrien A.","family":"Jump","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Donald","family":"Smiley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,8,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.47.4.265"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2478(89)90089-8"},{"key":"e_1_2_1_4_2","volume-title":"The origin of rheumatoid arthritis, Rheumatology","author":"Svartz N","year":"1975"},{"key":"e_1_2_1_5_2","first-page":"115","article-title":"Antibody to streptococcal cell wall peptidoglycan\u2010polysaccharide polymers in seropositive and seronegative rheumatic diseases","volume":"55","author":"Johnson PM","year":"1984","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.45.12.991"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1985.tb01931.x"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00270282"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI105758"},{"key":"e_1_2_1_10_2","first-page":"304","article-title":"In vivo synthesis of IgG by rheumatoid synovium","volume":"76","author":"Sliwinski AJ","year":"1970","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_11_2","first-page":"1","article-title":"Kappa:lambda light chain ratio in IgG eluted from rheumatoid arthritis synovium","volume":"7","author":"Lindstrom FD","year":"1970","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_1_12_2","first-page":"55","article-title":"Immunoglobulin classes, subclasses and complexes of IgG rheumatoid factor in rheumatoid plasma cells","volume":"12","author":"Munthe E","year":"1972","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI110424"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780270903"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780280704"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780251210"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(87)90324-3"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI105758"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780310302"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(60)90049-7"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(84)90034-6"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)63022-4"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/182670a0"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(89)90250-9"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(85)90303-5"},{"key":"e_1_2_1_27_2","doi-asserted-by":"crossref","first-page":"969","DOI":"10.4049\/jimmunol.133.2.969","article-title":"Purification and some properties of streptococcal protein G, a novel IgG\u2010binding reagent","volume":"133","author":"Bjorck L","year":"1984","journal-title":"J Immunol"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6122269"},{"key":"e_1_2_1_29_2","doi-asserted-by":"crossref","first-page":"3802","DOI":"10.4049\/jimmunol.135.6.3802","article-title":"A restricted human anti\u2010tetanus clonotype shares idiotypic cross\u2010reactivity with tetanus antibodies from most human donors and rabbits: reactivity with antibodies of widely differing electrophoretic mobility","volume":"135","author":"Hoffman WL","year":"1985","journal-title":"J Immunol"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(89)90740-9"}],"container-title":["Arthritis &amp; 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