{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T05:27:16Z","timestamp":1767677236322},"reference-count":42,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,12,9]],"date-time":"2005-12-09T00:00:00Z","timestamp":1134086400000},"content-version":"vor","delay-in-days":5336,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1991,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Anti\u2010Sm\u2013positive sera from patients with systemic lupus erythematosus (SLE) recognize a major epitope located within the carboxyl\u2010terminal 27 amino acids of a recombinant SmB fusion protein. To determine whether a synthetic peptide corresponding to this region could be used as an antigen to detect anti\u2010Sm antibodies, sera were typed as anti\u2010Sm positive or anti\u2010Sm negative by counterimmunoelectrophoresis (CIE). Twenty\u2010three SLE sera that were anti\u2010Sm positive by CIE, 22 that were anti\u2010Sm negative by CIE, and 42 sera from patients with other autoimmune diseases were tested for anti\u2010Sm antibodies by enzyme\u2010linked immunosorbent assay (ELISA), using either the synthetic peptide (C27) or a recombinant SmB (rSmB) fusion protein as the antigen. More than 90% of the sera that were anti\u2010Sm positive by CIE were also positive by both the C27 and rSmB ELISAs, and an additional 2 SLE sera originally typed as anti\u2010Sm negative were found to be positive (1 by the C27 ELISA, 1 by the rSmB ELISA), due to the greater sensitivity of the ELISAs. In the rSmB ELISA, anti\u2010Sm antibodies were not detected in any of the sera from patients with other autoimmune diseases, whereas 3 patients with anti\u2013U1 RNP antibodies (1 each with polymyositis, scleroderma, and mixed connective tissue disease) had a positive result in the C27 ELISA. These results indicate that both the C27 synthetic peptide and rSmB are excellent antigens for use in ELISAs to quantify anti\u2010Sm antibodies.<\/jats:p>","DOI":"10.1002\/art.1780340509","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T23:40:41Z","timestamp":1171496441000},"page":"572-579","source":"Crossref","is-referenced-by-count":26,"title":["Detection and quantification of human anti\u2010Sm antibodies using synthetic peptide and recombinant SmB antigens"],"prefix":"10.1002","volume":"34","author":[{"given":"John J.","family":"Hines","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keith 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