{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T16:55:06Z","timestamp":1765040106619},"reference-count":19,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,12,9]],"date-time":"2005-12-09T00:00:00Z","timestamp":1134086400000},"content-version":"vor","delay-in-days":5305,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1991,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have identified a novel antinuclear antibody (ANA) in the sera of patients with primary biliary cirrhosis (PBC). Sera from 22 of 50 PBC patients stained the nuclei of HEp\u20102 cells in a pattern of 5\u201320 discrete dots, on indirect immunofluorescence. Typically, the titers were higher than 1:1,280. This staining pattern was seen in only 4 of 1,240 ANA\u2010positive samples from patients with other connective tissue diseases, and in none of the ANA\u2010positive sera from 49 patients with other liver diseases. Sera which stained the nuclear dots bound a 95\u2010kd polypeptide in immunoblots of HeLa nuclei and in immunoprecipitates of <jats:sup>35<\/jats:sup>S\u2010methionine\u2013labeled HeLa extracts. Affinity\u2010purified antibodies from the 95\u2010kd protein reproduced the nuclear dot immunofluorescence staining pattern, demonstrating that this polypeptide is located in the nuclear dots. ANA of this type, therefore, occur in 44% of patients with PBC, and they appear to be specific for PBC. These antibodies bind a 95\u2010kd protein that is discrete and is scattered throughout the nucleus.<\/jats:p>","DOI":"10.1002\/art.1780340614","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T23:40:35Z","timestamp":1171496435000},"page":"731-736","source":"Crossref","is-referenced-by-count":33,"title":["PBC 95K, a 95\u2010kilodalton nuclear autoantigen in primary biliary cirrhosis"],"prefix":"10.1002","volume":"34","author":[{"given":"Janine","family":"Evans","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrian","family":"Reuben","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joe","family":"Craft","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,9]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-99-4-500"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(82)91327-7"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840080128"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(89)90088-1"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0098-2997(85)90007-X"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.4049\/jimmunol.135.3.1739","article-title":"Reactivity of anti\u2010mitochondrial autoantibodies in primary biliary cirrhosis: definition of two novel mitochondrial polypeptide autoantigens","volume":"135","author":"Frazer IH","year":"1985","journal-title":"J Immunol"},{"key":"e_1_2_1_8_2","volume-title":"Diseases of the intrahepatic bile ducts, Pathology of the Liver","author":"Portmann B","year":"1987"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113511"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)43211-5"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.94.3.749"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(84)90164-8"},{"key":"e_1_2_1_15_2","first-page":"553","article-title":"Diversity of autoantibodies in primary biliary cirrhosis and chronic active hepatitis","volume":"55","author":"Bernstein RM","year":"1984","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.38.7.801"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780270114"},{"key":"e_1_2_1_18_2","first-page":"108","article-title":"Autoimmune sera recognize a 100 kD nuclear protein antigen (sp\u2010100)","volume":"68","author":"Szostecki C","year":"1987","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_1_19_2","first-page":"283","article-title":"Autoantibodies to 200 kD polypeptide(s) of the nuclear envelope: a new serologic marker of primary biliary cirrhosis","volume":"74","author":"Lassoued K","year":"1988","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114696"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.1780340614","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.1780340614","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T16:53:48Z","timestamp":1700758428000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.1780340614"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,6]]},"references-count":19,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1991,6]]}},"alternative-id":["10.1002\/art.1780340614"],"URL":"https:\/\/doi.org\/10.1002\/art.1780340614","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,6]]}}}