{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T09:53:27Z","timestamp":1760608407658},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,12,9]],"date-time":"2005-12-09T00:00:00Z","timestamp":1134086400000},"content-version":"vor","delay-in-days":5091,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1992,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Objective.<\/jats:italic> Synovial fluid lymphocytes from patients with rheumatoid arthritis and with other forms of inflammatory synovitis demonstrate enhanced proliferative responses to <jats:italic>Mycobacterium tuberculosis<\/jats:italic> antigens, in particular, the 65\u2010kd heat\u2010shock protein. There is a high degree of homology between the human and the mycobacterial 60\u2010kd family of heat\u2010shock proteins. These studies were performed to determine if the enhanced response to the mycobacterial 65\u2010kd heat\u2010shock protein was due to cross\u2010reactivity of an immune response generated against the human homolog.<\/jats:p><jats:p><jats:italic>Methods.<\/jats:italic> These studies were performed by in vitro culture of isolated synovial fluid mononuclear cells with crude and purified antigens.<\/jats:p><jats:p><jats:italic>Results.<\/jats:italic> The synovial fluid lymphocytes of a majority of patients with rheumatoid arthritis recognized the mycobacterial 65\u2010kd heat\u2010shock protein, as evidenced by T cell proliferation. In contrast, only 18% of all samples tested responded to a highly purified recombinant human 60\u2010kd heat\u2010shock protein. With only one exception, proliferative responses to the mycobacterial antigen were stronger than those to the human homolog. The proliferative responses generated against mycobacterial 65\u2010kd heat\u2010shock proteins from different sources were highly correlated.<\/jats:p><jats:p><jats:italic>Conclusion.<\/jats:italic> The findings suggest that the enhanced proliferative response to the mycobacterial 65\u2010kd heat\u2010shock protein noted in most patients with rheumatoid arthritis and other forms of inflammatory synovitis is not due to cross\u2010reactivity of an immune response directed against the human heat\u2010shock protein.<\/jats:p>","DOI":"10.1002\/art.1780350107","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T23:49:17Z","timestamp":1171496957000},"page":"43-48","source":"Crossref","is-referenced-by-count":41,"title":["Activation of Synovial Fluid T Lymphocytes by 60\u2010Kd Heat\u2010Shock Proteins in Patients with Inflammatory synovitis"],"prefix":"10.1002","volume":"35","author":[{"given":"Richard 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