{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T23:02:12Z","timestamp":1791068532486,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2005,12,9]],"date-time":"2005-12-09T00:00:00Z","timestamp":1134086400000},"content-version":"vor","delay-in-days":4909,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1992,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Objective.<\/jats:italic> We have previously described anti\u2010EJ antibodies, and provided evidence that these antibodies react with glycyl\u2013transfer RNA (gly\u2010tRNA) synthetase. The aim of the present study was to identify patients with anti\u2010EJ antibodies and describe the clinical associations of the antibody, in particular, whether it is associated with the syndrome of myositis and interstitial lung disease (ILD) that has been previously associated with autoantibodies to the aminoacyl\u2010tRNA synthetases for histidine, threonine, and alanine.<\/jats:p><jats:p><jats:italic>Methods.<\/jats:italic> Sera from patients with suspected or proven polymyositis or dermatomyositis (DM), sera with anticytoplasmic patterns, and control sera were tested for anti\u2010EJ antibodies by immunoprecipitation (IPP). Positive sera and controls were tested for the ability to inhibit gly\u2010tRNA synthetase by preincubation of the enzyme source with the serum.<\/jats:p><jats:p><jats:italic>Results.<\/jats:italic> Anti\u2010EJ antibodies were demonstrated in the sera of 5 patients, by IPP of characteristic tRNAs and protein. Original serum EJ and each of the new sera significantly inhibited the enzymatic activity of gly\u2010tRNA synthetase but not histidyl\u2010tRNA synthetase. All 5 of the new patients had inflammatory myopathy, a typical DM rash, and ILD. One, who had an overlap syndrome with systemic lupus erythematosus, had anti\u2010EJ at least 4 months before the development of clinical myositis. Arthritis and Raynaud's phenomenon, other features associated with antisynthetases, were also seen.<\/jats:p><jats:p><jats:italic>Conclusion.<\/jats:italic> Anti\u2010EJ is associated with the syndrome of myositis and lung disease that is seen in association with other antisynthetases. The finding of specific inhibition of gly\u2010tRNA synthetase by all anti\u2010EJ\u2013positive sera strongly supports the identification of EJ antigen as gly\u2010tRNA synthetase.<\/jats:p>","DOI":"10.1002\/art.1780350718","type":"journal-article","created":{"date-parts":[[2007,2,15]],"date-time":"2007-02-15T00:13:33Z","timestamp":1171498413000},"page":"821-830","source":"Crossref","is-referenced-by-count":90,"title":["Antibodies to glycyl\u2013transfer rna synthetase in patients with myositis and interstitial lung disease"],"prefix":"10.1002","volume":"35","author":[{"given":"Ira N.","family":"Targoff","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward P.","family":"Trieu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul H.","family":"Plotz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederick W.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,12,9]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/304177a0"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.160.2.420"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.163.5.1281"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.4049\/jimmunol.144.5.1737","article-title":"Autoantibodies to aminoacyl\u2010transfer RNA synthetases for isoleucine and glycine: two additional synthetases are antigenic in myositis","volume":"144","author":"Targoff IN","year":"1990","journal-title":"J Immunol"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-199111000-00002"},{"key":"e_1_2_1_7_2","first-page":"487","article-title":"Humoral immunity in polymyositis and dermatomyositis","volume":"55","author":"Targoff IN","year":"1988","journal-title":"Mt Sinai J Med"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"2874","DOI":"10.4049\/jimmunol.138.9.2874","article-title":"Measurement of antibody to Jo\u20101 by ELISA and comparison to enzyme inhibitory activity","volume":"138","author":"Targoff IN","year":"1987","journal-title":"J Immunol"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(87)90011-1"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780260505"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.289.6438.151"},{"key":"e_1_2_1_12_2","first-page":"663","article-title":"Antibody to Jo\u20101 in polymyositis\/dermatomyositis: association with interstitial pulmonary disease","volume":"11","author":"Hochberg MC","year":"1984","journal-title":"J Rheumatol"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780310408"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(90)90149-8"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780230802"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780331106"},{"key":"e_1_2_1_17_2","volume-title":"Jo\u20101 and other myositis autoantibodies, Rheumatology\u201085. Excerpta Medica International Congress Series","author":"Bernstein RM","year":"1985"},{"issue":"9","key":"e_1_2_1_18_2","first-page":"S134","article-title":"Reaction of anti\u2010OJ with components of the multi\u2010enzyme synthetase complex (abstract)","volume":"34","author":"Targoff IN","year":"1991","journal-title":"Arthritis Rheum"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197502132920706"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197502202920807"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780280711"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780330908"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780281207"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780251102"},{"issue":"9","key":"e_1_2_1_25_2","first-page":"S52","article-title":"Failure of plasma exchange and leukapheresis to improve polymyositis or dermatomyositis: the results of a double\u2010blind, randomized, sham\u2010controlled trial (abstract)","volume":"34","author":"Miller FW","year":"1991","journal-title":"Arthritis Rheum"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-111-2-143"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(87)90308-5"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0049-0172(88)90032-7"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.24.9933"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780311215"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.2.8.2452112"},{"issue":"4","key":"e_1_2_1_32_2","first-page":"S22","article-title":"Comparison of species specificity of autoimmune anti\u2010Jo\u20101 and induced rabbit anti\u2010Jo\u20101 (abstract)","volume":"32","author":"Roberts MM","year":"1989","journal-title":"Arthritis Rheum"},{"key":"e_1_2_1_33_2","first-page":"21","article-title":"Two human tRNAala families are recognized by autoantibodies in polymyositis sera","volume":"4","author":"Bunn CC","year":"1987","journal-title":"Mol Biol Med"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2446387"},{"key":"e_1_2_1_35_2","volume-title":"Immunological aspects, Inflammatory Diseases of Muscle","author":"Targoff IN","year":"1988"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114136"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(83)90740-7"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.1780350718","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.1780350718","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T07:16:17Z","timestamp":1700723777000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.1780350718"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,7]]},"references-count":36,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1992,7]]}},"alternative-id":["10.1002\/art.1780350718"],"URL":"https:\/\/doi.org\/10.1002\/art.1780350718","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,7]]}}}