{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T05:56:29Z","timestamp":1791006989217,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,12,9]],"date-time":"2005-12-09T00:00:00Z","timestamp":1134086400000},"content-version":"vor","delay-in-days":4725,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1993,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Objective.<\/jats:italic> To investigate the involvement of phospholipase D in the signaling pathways activated by 2 pathologically relevant inflammatory microcrystals, monosodium urate (MSU) and calcium pyrophosphate dihydrate (CPPD).<\/jats:p><jats:p><jats:italic>Methods.<\/jats:italic> Human peripheral blood neutrophils were used throughout. Phospholipase D activity was monitored by measuring 3 separate indices: 1) the mass of phosphatidic acid, 2) the levels of alkyl\u2010phosphatidic acid, and 3) the levels of formation, in the presence of ethanol, of phosphatidylethanol. The latter 2 parameters were measured in cells labeled with 1\u20100\u2010<jats:sup>3<\/jats:sup>H\u2013alkyl\u20102\u2010acetyl\u2010sn\u2010glycero\u20103\u2010phosphocholine. The cells were stimulated with microcrystals of triclinic morphology.<\/jats:p><jats:p><jats:italic>Results.<\/jats:italic> Both MSU and CPPD crystals induced a time\u2010 and concentration\u2010dependent accumulation of phosphatidic acid mass and elevation in levels of alkyl\u2010phosphatidic acid and phosphatidylethanol in prelabeled cells. The activation of phospholipase D by the microcrystals was partially sensitive to colchicine and largely resistant to pertussis toxin. Inhibition of phosphatidic acid formation by wortmannin or ethanol reduced the microcrystal\u2010stimulated production of superoxide anions.<\/jats:p><jats:p><jats:italic>Conclusion.<\/jats:italic> These results indicate that microcrystals stimulate phospholipase D in human neutrophils and that at least some of the functional consequences of neutrophil\u2010microcrystal interactions may be dependent on this biochemical pathway.<\/jats:p>","DOI":"10.1002\/art.1780360119","type":"journal-article","created":{"date-parts":[[2007,2,15]],"date-time":"2007-02-15T00:54:59Z","timestamp":1171500899000},"page":"117-125","source":"Crossref","is-referenced-by-count":25,"title":["Crystal\u2010induced neutrophil activation. II. evidence for the activation of a phosphatidylcholine\u2010specific phospholipase D"],"prefix":"10.1002","volume":"36","author":[{"given":"Paul H.","family":"Naccache","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvain","family":"Bourgoin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edith","family":"Plante","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles J.","family":"Roberge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"De Medicis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9","family":"Lussier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice 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