{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T08:26:37Z","timestamp":1791620797646,"version":"4.3.4"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,12,9]],"date-time":"2005-12-09T00:00:00Z","timestamp":1134086400000},"content-version":"vor","delay-in-days":4605,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1993,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Objective.<\/jats:italic> Previous studies suggested that it was possible to characterize the intact surface of articular cartilage by probing it with antibodies against matrix macromolecules. The present studies were undertaken to investigate type II collagen (CII) epitope availability on the intact surface of articular cartilage.<\/jats:p><jats:p><jats:italic>Methods.<\/jats:italic> Normal bovine, rabbit, and human cartilage specimens were used to measure binding of anti\u2010CII antibodies to the articular and cut surfaces of cartilage. Antisera were raised against the material obtained after brief extraction of the cartilage surface with 4<jats:italic>M<\/jats:italic> guanidine solution.<\/jats:p><jats:p><jats:italic>Results.<\/jats:italic> Anti\u2010CII did not bind to the intact surface of rabbit articular cartilage when compared with control rat sera, but did bind significantly to the cut surface. With normal human articular cartilage, the cut surfaces bound approximately 4 times as much anti\u2010CII antibody as the intact articular surfaces. These findings suggested that the CII epitopes are normally protected by the superficial cartilage layer. In experiments carried out to characterize this layer, binding of anti\u2010CII antibodies was unchanged after exhaustive washing of bovine or rabbit cartilage with phosphate buffered saline or 1<jats:italic>M<\/jats:italic> NaCl solution, whereas it was significantly increased after brief exposure to 4<jats:italic>M<\/jats:italic> guanidine solution or after incubation with neutrophil elastase. No restoration of the protection of CII epitopes in guanidine\u2010treated cartilage could be achieved by incubation with undiluted normal bovine synovial fluid; however, 8\u2010day culture of elastase\u2010treated cartilage explants resulted in partial restoration of protection of the CII epitopes. Rat and rabbit antisera raised against the cartilage surface material extracted with 4<jats:italic>M<\/jats:italic> guanidine contained antibodies that bound to the cartilage surface. By Western blotting, rat antibodies identified 50\u201365\u2010kd protein bands present in the guanidine extract, but not present either in the material obtained from brief digestion of cartilage with neutrophil elastase or in synovial fluid. The rabbit antisera identified a broad 70\u201395\u2010kd band. Exposure of elastase\u2010treated cartilage to the guanidine\u2010extracted material resulted in a partial decrease of anti\u2010CII antibody binding.<\/jats:p><jats:p><jats:italic>Conclusion.<\/jats:italic> These results suggest that CII on intact cartilage is protected from antibody binding, and that the protective material is at least partly proteinaceous in nature, is unlikely to be derived from synovial fluid, is noncovalently bound to the underlying intercellular matrix, and is synthesized by resident chondrocytes. Further characterization of the protective material may provide important information on the mechanisms of early cartilage damage in inflammatory arthritis.<\/jats:p>","DOI":"10.1002\/art.1780360512","type":"journal-article","created":{"date-parts":[[2007,2,15]],"date-time":"2007-02-15T00:40:02Z","timestamp":1171500002000},"page":"651-659","source":"Crossref","is-referenced-by-count":24,"title":["Characteristics of anti\u2010type ii collagen antibody binding to articular cartilage"],"prefix":"10.1002","volume":"36","author":[{"given":"Hugo E.","family":"Jasin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koji","family":"Noyori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshitaka","family":"Takagi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joel D.","family":"Taurog","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,12,9]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"251","article-title":"The movements of bone and joints. IV. The mechanical structure of articulating cartilage","volume":"33","author":"MacConaill MA","year":"1951","journal-title":"J Bone Joint Surg [Br]"},{"key":"e_1_2_1_3_2","volume-title":"Osteoarthritis, Bones and Joints","author":"Sokoloff L","year":"1976"},{"key":"e_1_2_1_4_2","volume-title":"Biology of cartilage cells, Biological Structure and Function","author":"Stockwell RA","year":"1979"},{"key":"e_1_2_1_5_2","volume-title":"Fine Structure of Synovial Joints","author":"Ghadially FN","year":"1983"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-196850040-00002"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01003221"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"388","DOI":"10.2106\/00004623-198264030-00009","article-title":"The negative charge of articular cartilage surfaces: an electron microscopic study using cationized ferritin","volume":"64","author":"Stanescu R","year":"1982","journal-title":"J Bone Joint Surg [Am]"},{"key":"e_1_2_1_9_2","first-page":"833","article-title":"Effects of enzymatic digestions on the negative charge of articular cartilage surfaces","volume":"12","author":"Stanescu R","year":"1985","journal-title":"J Rheumatol"},{"key":"e_1_2_1_10_2","first-page":"279","article-title":"Repartition differente du collagene de type I et du collagene de type II dans la zone superficielle et dans la zone intermediaire du cartilage articulaire","volume":"283","author":"Stanescu V","year":"1976","journal-title":"C R Acad Sci [III]"},{"key":"e_1_2_1_11_2","volume-title":"Articular cartilage morphology, Articular Cartilage Biochemistry","author":"Schenk RK","year":"1986"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115164"},{"key":"e_1_2_1_13_2","doi-asserted-by":"crossref","first-page":"6469","DOI":"10.1016\/S0021-9258(19)44716-9","article-title":"Collagen heterogeneity","volume":"247","author":"Trelstad RL","year":"1973","journal-title":"J Biol Chem"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI110290"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780280302"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/anr.1780320302"},{"issue":"14","key":"e_1_2_1_17_2","first-page":"25","article-title":"A growing family of collagens in articular cartilage: identification of 5 genetically distinct types","volume":"14","author":"Eyre DR","year":"1987","journal-title":"J Rheumatol"},{"issue":"1","key":"e_1_2_1_18_2","first-page":"S74","article-title":"Identification of a proteoglycan on the articular cartilage surface that interferes with anti\u2010type II collagen antibody binding (abstract)","volume":"35","author":"Noyori K","year":"1992","journal-title":"Arthritis Rheum"},{"key":"e_1_2_1_19_2","volume-title":"Immunization procedures, isolation by affinity chromatography, and serological and immunochemical characterization of collagen specific antibodies, Immunochemistry of the Extracellular Matrix","author":"Furthmeyr H","year":"1982"},{"key":"e_1_2_1_20_2","first-page":"360A","article-title":"Repair characteristics of the articular cartilage surface following acute inflammation (abstract)","volume":"40","author":"Noyori K","year":"1992","journal-title":"Clin Res"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.3.9.2663581"},{"key":"e_1_2_1_22_2","volume-title":"Type VI collagen, Structure and Function of Collagen Types","author":"Timpl R","year":"1987"},{"key":"e_1_2_1_23_2","volume-title":"Fibronectin domains and receptors, Fibronectin","author":"Yamada KM","year":"1989"},{"key":"e_1_2_1_24_2","doi-asserted-by":"crossref","first-page":"6898","DOI":"10.1016\/S0021-9258(18)83516-5","article-title":"Interactions of a 59\u2010kDa connective tissue matrix protein with collagen I and collagen II","volume":"264","author":"Hedbom E","year":"1982","journal-title":"J Biol Chem"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(85)90077-5"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780240104"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1302\/0301-620X.52B3.554"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780290605"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780241104"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780340621"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780340514"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.1780360512","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.1780360512","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T04:28:07Z","timestamp":1700713687000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.1780360512"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,5]]},"references-count":30,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1993,5]]}},"alternative-id":["10.1002\/art.1780360512"],"URL":"https:\/\/doi.org\/10.1002\/art.1780360512","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,5]]}}}