{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T10:58:55Z","timestamp":1760266735697},"reference-count":40,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2005,12,9]],"date-time":"2005-12-09T00:00:00Z","timestamp":1134086400000},"content-version":"vor","delay-in-days":4482,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1993,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Objective.<\/jats:italic> To evaluate the possibility that lymphoproliferation in primary Sj\u00f6gren's syndrome (SS) arises within a subset of B cells.<\/jats:p><jats:p><jats:italic>Methods.<\/jats:italic> A panel of monoclonal antibodies (MAb) specific for rheumatoid factor (RF)\u2013associated cross\u2010reactive idiotypes (CRI) and anti\u2010V<jats:sub>K<\/jats:sub> and anti\u2010V<jats:sub>H<\/jats:sub> subgroup antibodies were used to define the clonality of B lymphocytes undergoing neoplastic transformation in 5 patients with primary SS. Anti\u2010CRI antibodies were also used to study longitudinal variations in serum paraprotein levels and in vitro regulation of IgM and IgM\u2010RF production by peripheral blood lymphocytes. The levels of CRI, IgM, and IgM\u2010RF were quantitated in serum and culture supernatants by enzyme\u2010linked immunosorbent assay. Heavy and light chain isotypes and V<jats:sub>H<\/jats:sub> subgroups of the paraproteins were determined by immunoelectrophoresis, immunofixation, and Western blotting.<\/jats:p><jats:p><jats:italic>Results.<\/jats:italic> Paraproteins from all patients expressed an epitope associated with V<jats:sub>K<\/jats:sub>IIIb sub\u2010subgroup of light chains. Three of the paraproteins were cryoglobulins with RF activity, all of which expressed the V<jats:sub>K<\/jats:sub>IIIb\u2010associated CRI (detected by MAb 17\u2013109) and the V<jats:sub>H<\/jats:sub>I\u2010associated CRI (detected by MAb G6 and G8). None of the paraproteins expressed the V<jats:sub>H<\/jats:sub>III\u2010associated CRI (detected by MAb B6 and D12). The CRI were consistently expressed over a period of 5\u20136 years. The anti\u2010CRI and anti\u2010subgroup antibodies substantially inhibited spontaneous production of IgM\u2010RF and IgM by peripheral blood B lymphocytes from 3 of the SS patients.<\/jats:p><jats:p><jats:italic>Conclusion.<\/jats:italic> These results suggest that lymphoproliferation in primary SS is highly selective, and that the anti\u2010CRI antibodies can be used as an aid to early diagnosis as well as for monitoring and modulating the lymphoproliferative process in primary SS.<\/jats:p>","DOI":"10.1002\/art.1780360814","type":"journal-article","created":{"date-parts":[[2007,2,15]],"date-time":"2007-02-15T01:08:49Z","timestamp":1171501729000},"page":"1128-1136","source":"Crossref","is-referenced-by-count":26,"title":["Lymphoproliferation in primary sj\u00f6gren's syndrome. evidence of selective expansion of a b cell subset characterized by the expression of cross\u2010reactive idiotypes"],"prefix":"10.1002","volume":"36","author":[{"given":"Fazel","family":"Shokri","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rizgar A.","family":"Mageed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Robert Maziak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norman","family":"Talal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas","family":"Amos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bryan D.","family":"Williams","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roy","family":"Jefferis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,9]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"19","article-title":"The Copenhagen criteria for Sj\u00f6gren's syndrome","volume":"61","author":"Manthorpe R","year":"1986","journal-title":"Scand J Rheumatol Suppl"},{"key":"e_1_2_1_3_2","first-page":"118","article-title":"Lymphoproliferative disorders in patients with Sj\u00f6gren's syndrome","volume":"61","author":"Sugai S","year":"1986","journal-title":"Scand J Rheumatol Suppl"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780290907"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-89-6-888"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.45.3.210"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198704303161803"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-8411(88)90061-3"},{"key":"e_1_2_1_9_2","unstructured":"SugaiS ShimizuS TachibanaJ KundaS:The high incidence of rheumatoid factor idiotypes in monoclonal Igs associated with Sj\u00f6gren's syndrome The Second International Conference on Sj\u00f6gren's syndrome. 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