{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:57:28Z","timestamp":1790647048389,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2005,12,13]],"date-time":"2005-12-13T00:00:00Z","timestamp":1134432000000},"content-version":"vor","delay-in-days":4486,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis & Rheumatism"],"published-print":{"date-parts":[[1993,9]]},"abstract":"Abstract<\/jats:title>Objective.<\/jats:italic> In this in vivo study, we investigated changes in the proteins that coat monosodium urate (MSU) crystals in human synovial fluid samples and rat air pouch fluid samples obtained sequentially during periods of active and resolving inflammation, in order to evaluate whether in vivo findings are consistent with hypotheses on roles of protein coating based on in vitro findings.<\/jats:p>Methods.<\/jats:italic> Crystals from patients with gout were isolated from joint fluids with acute inflammation, and subsequently from the same joints at the time inflammation was resolving. Crystals were also obtained using the rat subcutaneous air pouch model. Immunogold was used to label proteins coating MSU crystals, for light microscopy (LM) and transmission electron microscopy (TEM) studies.<\/jats:p>Results.<\/jats:italic> Dense immunogold\u2013silver labeling for IgG was observed under LM on crystals from fluid with acute inflammation, whereas other proteins (apolipoproteins [Apo], fibronectin, fibrinogen, albumin) were not labeled significantly. Apo B became strongly positive on crystals as the inflammation subsided, whereas other proteins were only weakly positive and IgG became absent or weakly positive. Quantitative TEM evaluation confirmed the LM observations.<\/jats:p>Conclusion.<\/jats:italic> This study provides the first in vivo evidence supporting the notion derived from previous in vitro studies that proteins coating MSU crystals change as inflammation evolves. Protein coatings may play an important role in the self\u2010limited nature of gouty inflammation. IgG coating MSU crystals may enhance the inflammation. As the inflammation subsides, Apo B could displace the IgG by competitively coating sites on crystals and could contribute in part to the resolution of the acute gouty arthritis.<\/jats:p>","DOI":"10.1002\/art.1780360912","type":"journal-article","created":{"date-parts":[[2007,2,15]],"date-time":"2007-02-15T01:08:23Z","timestamp":1171501703000},"page":"1274-1285","source":"Crossref","is-referenced-by-count":85,"title":["Changes in the proteins coating monosodium urate crystals during active and subsiding inflammation. immunogold studies of synovial fluid from patients with gout and of fluid obtained using the rat subcutaneous air pouch model"],"prefix":"10.1002","volume":"36","author":[{"given":"Esteban","family":"Ortiz\u2010Bravo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie S.","family":"Sieck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"H.","family":"Ralph Schumacher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,12,13]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"Textbook of Rheumatology","author":"Kelley WN","year":"1989"},{"key":"e_1_2_1_3_2","volume-title":"Arthritis and Allied Conditions","author":"Terkeltaub RA","year":"1989"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-91-1-56"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780291209"},{"key":"e_1_2_1_6_2","first-page":"967","article-title":"Monosodium urate crystals in asymptomatic knee joints","volume":"9","author":"Gordon TP","year":"1982","journal-title":"J Rheumatol"},{"key":"e_1_2_1_7_2","first-page":"49","article-title":"Molecular orientation of immunoglobulin G adsorbed to microcrystalline monosodium urate monohydrate","volume":"95","author":"Kozin F","year":"1980","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_8_2","first-page":"519","article-title":"Polymorphonuclear leukocyte response to monosodium urate crystals: modification by absorbed serum proteins","volume":"6","author":"Kozin F","year":"1979","journal-title":"J Rheumatol"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780250210"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780291208"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780260612"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780290309"},{"key":"e_1_2_1_13_2","volume-title":"Atlas of Synovial Fluid Analysis and Crystal Identification","author":"Schumacher HR","year":"1991"},{"key":"e_1_2_1_14_2","volume-title":"Techniques in Immunocytochemistry","author":"Roth J","year":"1983"},{"key":"e_1_2_1_15_2","doi-asserted-by":"crossref","unstructured":"SelyeH:Induction of topical resistance to acute tissue injury. 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