{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T07:53:37Z","timestamp":1761292417242},"reference-count":17,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,12,9]],"date-time":"2005-12-09T00:00:00Z","timestamp":1134086400000},"content-version":"vor","delay-in-days":4329,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1994,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Objective<\/jats:italic>. To evaluate an enzyme\u2010linked immunosorbent assay (ELISA) for anticentromere autoantibodies (ACA).<\/jats:p><jats:p><jats:italic>Methods<\/jats:italic>. Sera from 611 patients with scleroderma, CREST (calcinosis, Raynaud's phenomenon, esophageal dysmotility, sclerodactyly, telangiectasias), Raynaud's disease, and connective tissue disease control patients were studied by ELISA using the fusion protein CENP\u2010B, by immunofluorescence on dividing HEp\u20102 cells, and by immunoblotting on chromosomes and CENP\u2010B.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>. Compared with immunofluorescence, the CENP\u2010B ELISA sensitivity was 94% and the specificity was 93%. In 19.7% of the cases, there was a probability of a false\u2010positive result and in 1.9%, a probability of a false\u2010negative result, yielding positive and negative predictive values of 0.80 and 0.98, respectively.<\/jats:p><jats:p><jats:italic>Conclusion<\/jats:italic>. The CENP\u2010B ELISA is a sensitive and specific assay for ACA.<\/jats:p>","DOI":"10.1002\/art.1780370214","type":"journal-article","created":{"date-parts":[[2007,2,15]],"date-time":"2007-02-15T00:56:34Z","timestamp":1171500994000},"page":"248-252","source":"Crossref","is-referenced-by-count":20,"title":["Anticentromere Autoantibodies"],"prefix":"10.1002","volume":"37","author":[{"given":"Dolores","family":"V\u00e1zquez\u2010Abad","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan","family":"Wallace","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Luc","family":"Sen\u00e9cal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"France","family":"Joyal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andre","family":"Roussin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William C.","family":"Earnshaw","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naomi","family":"Rothfield","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,9]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.3.1627"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780230602"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.41.4.382"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00328227"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.104.4.817"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780301214"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780230510"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780310302"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780251101"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780290501"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-197707000-00001"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780340111"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780310309"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.32.4.337"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/anr.1780320213"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116704"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.292.6522.746"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.1780370214","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.1780370214","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T19:10:31Z","timestamp":1700766631000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.1780370214"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,2]]},"references-count":17,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,2]]}},"alternative-id":["10.1002\/art.1780370214"],"URL":"https:\/\/doi.org\/10.1002\/art.1780370214","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,2]]}}}