{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T10:51:09Z","timestamp":1791024669859,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,12,9]],"date-time":"2005-12-09T00:00:00Z","timestamp":1134086400000},"content-version":"vor","delay-in-days":4209,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1994,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Objective. In subjects with idiopathic calcium pyrophosphate dihydrate (CPPD) deposition disease, cartilage chondrocytes elaborate increased amounts of PP<jats:sub>i<\/jats:sub>. The mechanism of the intracellular PP<jats:sub>i<\/jats:sub> elevation is not known. Plasma membrane 5'\u2010nucleotide phosphodiesterase I\/nucleotide pyrophosphohydrolase (NTPPPH) activity also is elevated in chondrocytes and dermal fibroblasts of patients with idiopathic CPPD deposition disease. NTPPPH, as an ecto\u2010enzyme, could act within certain intracellular compartments. Thus, we hypothesized a potential causal link between increased NTPPPH activity and increased intracellular PP<jats:sub>i<\/jats:sub>.\nMethods. Transformed simian fibroblasts (COS cells) and human osteoblasts (U2OS cells) were transfected with the 5'\u2010nucleotide phosphodiesterase I ectoenzyme plasma cell membrane glycoprotein\u20131 (PC\u20101), recently shown to be expressed in cartilage, osteoblasts, and fibroblasts.\nResults. Transfection with PC\u20101 markedly upregulated 5'\u2010nucleotode phosphodiesterase I activity and increased intracellular PP<jats:sub>i<\/jats:sub> concentrations by increasing the capacity of cells to generate PP<jats:sub>i<\/jats:sub>. Importantly, this did not require supplementation with exogenous nucleotides.\nConclusion. Cellular overexpression of PC\u20101 produces NTPPPH overactivity and increased intracellular PP<jats:sub>i<\/jats:sub> generation in vitro. These findings support the potential importance of NTPPPH overactivity in P<jats:sub>i<\/jats:sub> generation, both inside and outside the cell, in some subjects with CPPD deposition disease.<\/jats:p>","DOI":"10.1002\/art.1780370624","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T20:12:54Z","timestamp":1171483974000},"page":"934-941","source":"Crossref","is-referenced-by-count":75,"title":["Causal link between nucleotide pyrophosphohydrolase overactivity and increased intracellular inorganic pyrophosphate generation demonstrated by transfection of cultured fibroblasts and osteoblasts with plasma cell membrane glycoprotein\u20131"],"prefix":"10.1002","volume":"37","author":[{"given":"Robert","family":"Terkeltaub","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Rosenbach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felicia","family":"Fong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Goding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,12,9]]},"reference":[{"key":"e_1_2_1_1_2","volume-title":"Calcium pyrophosphate crystal deposition disease, pseudogout, articular chondrocalcinosis, Arthritis and Allied Conditions","author":"Ryan LM","year":"1993"},{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780341014"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/S0889-857X(21)00965-0","article-title":"Inorganic pyrophosphate metabolism in arthritis","volume":"14","author":"Rachow JW","year":"1989","journal-title":"Rheum Dis Clin North Am"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(92)90406-Y"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780240307"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780270210"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780270211"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112487"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(87)90023-7"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(74)90241-0"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1093\/clinchem\/23.9.1531","article-title":"Nucleotide pyrophosphatase and phosphodiesterase. 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