{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:59:17Z","timestamp":1791291557424,"version":"4.3.0"},"reference-count":27,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2005,12,9]],"date-time":"2005-12-09T00:00:00Z","timestamp":1134086400000},"content-version":"vor","delay-in-days":3661,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1995,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Objective<\/jats:italic>. To describe the histopathologic and biochemical characteristics of skin from the <jats:italic>Tsk2\/ +<\/jats:italic> mouse, a mutation with phenotypic features resembling those of systemic sclerosis (SSc), and to report the initial genetic mapping of the <jats:italic>Tsk2<\/jats:italic> locus.<\/jats:p><jats:p><jats:italic>Methods<\/jats:italic>. Histologic examination was performed and collagen content and type I collagen messenger RNA (mRNA) levels were determined in skin biopsy specimens from <jats:italic>Tsk2\/+<\/jats:italic> mice and normal mice. An intersubspecific backcross was conducted as a first step toward identifying the position of the <jats:italic>Tsk2<\/jats:italic> locus on mouse chromosome 1.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>. Histologic examination of <jats:italic>Tsk2\/+<\/jats:italic> mouse skin revealed marked accumulation of collagen and infiltration with mononuclear cells in the dermis and adipose tissue. Biochemical studies of <jats:italic>Tsk2\/+<\/jats:italic> mouse skin showed increased collagen content and elevated steady\u2010state levels of \u03b11(I) procollagen mRNA. <jats:italic>Tsk2<\/jats:italic> was mapped to a 15.3\u2010c entimorgan interval on mouse chromosome 1.<\/jats:p><jats:p><jats:italic>Conclusion. Tsk2<\/jats:italic> is a novel mutation which displays histopathologic and biochemical abnormalities similar to those present in the skin of patients with SSc, including increased collagen content and expression of type I collagen genes. This mutation has been mapped to a 15.3\u2010c M region on mouse chromosome 1. Further study of this novel mutation will allow the identification of previously undescribed mechanisms involved in the regulation of normal and pathologic collagen gene expression.<\/jats:p>","DOI":"10.1002\/art.1780381212","type":"journal-article","created":{"date-parts":[[2007,2,15]],"date-time":"2007-02-15T01:46:09Z","timestamp":1171503969000},"page":"1791-1798","source":"Crossref","is-referenced-by-count":89,"title":["The tight skin 2 mouse"],"prefix":"10.1002","volume":"38","author":[{"given":"Paul J.","family":"Christner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josephine","family":"Peters","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Hawkins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda D.","family":"Siracusa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio A.","family":"Jim\u00e9nez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,12,9]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"Systemic sclerosis (scleroderma), localized scleroderma, eosinophilic fasciitis, and calcinosis. 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