{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T13:05:24Z","timestamp":1771333524490,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,12,12]],"date-time":"2005-12-12T00:00:00Z","timestamp":1134345600000},"content-version":"vor","delay-in-days":3633,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1996,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Objective<\/jats:italic>. To elucidate the clinical significance and immunologic heterogeneity of anti\u2013glycyl\u2013transfer RNA (tRNA) synthetase antibodies in polymyositis\/dermatomyositis (PM\/DM).<\/jats:p><jats:p><jats:italic>Methods<\/jats:italic>. Sera from 345 patients with rheumatic diseases, including 91 with myositis, were examined using immunoprecipitation assays. Autoantibodies to aminoacyl\u2010tRNA synthetases were further analyzed with 2\u2010dimensional RNA fractionation and via inhibition of in vitro aminoacylation.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>. Serum from 1 patient with DM and interstitial lung disease immunoprecipitated glycyl\u2010tRNA synthetase along with only 1 of 4 associated tRNAs, in comparison with control anti\u2013glycyl\u2010tRNA synthetase antibodies, which bound the enzyme along with all 4 associated tRNAs. Immunoblotting findings and a lack of in vitro inhibition aminoacylation of tRNA<jats:sup>gly<\/jats:sup> by serum from this patient also suggested differences between the epitope specificity of this serum and that of other sera with anti\u2013glycyl\u2010tRNA synthetase antibodies.<\/jats:p><jats:p><jats:italic>Conclusion<\/jats:italic>. This identification of antibodies to glycyl\u2010tRNA synthetase from a patient with DM underscores the association of this specificity with the disease. The finding that these antibodies bound an epitope outside the active site of the synthetase enzyme, in contrast to most anti\u2013aminoacyl\u2010tRNA synthetases, emphasizes the immunologic heterogeneity of these autoantibodies.<\/jats:p>","DOI":"10.1002\/art.1780390119","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T11:53:26Z","timestamp":1171454006000},"page":"146-151","source":"Crossref","is-referenced-by-count":24,"title":["Autoantibodies to glycyl\u2013transfer RNA synthetase in myositis. 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