{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:57:28Z","timestamp":1790647048353,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2005,12,12]],"date-time":"2005-12-12T00:00:00Z","timestamp":1134345600000},"content-version":"vor","delay-in-days":3451,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1996,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Objective<\/jats:italic>. We investigated the effects of transforming growth factor \u03b21 (TGF\u03b21) on monosodium urate monohydrate (MSU) crystal\u2010induced acute inflammation in vivo.<\/jats:p><jats:p><jats:italic>Methods<\/jats:italic>. One hour after MSU crystal\u2013induced acute inflammation was produced in the rat subcutaneous air pouch model, the effects of recombinant human TGF\u03b21 (rHuTGF\u03b21; 10\u2013100 pg\/animal) and ultrapure TGF\u03b21 (UPTGF\u03b21; 100 and 500 pg\/animal) were assessed, based on absolute and differential white blood cell counts in the exudate. The effects of 10 pg of rHuTGF\u03b21 preincubated with a specific anti\u2010TGF\u03b2 antibody, and the effects of coinjection of crystals and rHuTGF\u03b21, were also studied.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>. UPTGF\u03b21 and rHuTGF\u03b21 markedly reduced MSU crystal\u2013induced inflammation. Recombinant human TGF\u03b21 also reduced inflammation when administered concomitantly with MSU crystals. Moreover, rHuTGF\u03b21 and UPTGF\u03b21, injected 1 hour after MSU crystal injection, reduced the inflammatory response in a dose\u2010dependent manner. Injection of rHuTGF\u03b21 (100 pg\/animal) resulted in a &gt;90% reduction in the maximal white blood cell count, achieved 6 hours after crystal injection. Preincubation of rHuTGF\u03b21 with a specific anti\u2010TGF\u03b21 antibody significantly (<jats:italic>P<\/jats:italic> &lt; 0.01) reversed the inhibitory effect of rHuTGF\u03b21 on the inflammatory response. Consistent with the regulation of inflammatory cell recruitment into the joint, the percentage of monocytes markedly decreased (<jats:italic>P<\/jats:italic> &lt; 0.01) following local injection with rHuTGF\u03b21 6 hours after MSU crystal injection.<\/jats:p><jats:p><jats:italic>Conclusion<\/jats:italic>. Exogenous TGF\u03b21 prevents and inhibits MSU crystal\u2013induced acute inflammation in vivo. Its role in the self\u2010limitation of gouty attacks deserves consideration, among the various other factors involved.<\/jats:p>","DOI":"10.1002\/art.1780390718","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T12:06:32Z","timestamp":1171454792000},"page":"1192-1198","source":"Crossref","is-referenced-by-count":68,"title":["Inhibition and prevention of monosodium urate monohydrate crystal\u2013induced acute inflammation in vivo by transforming growth factor 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