{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T16:09:21Z","timestamp":1764173361418},"reference-count":39,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2005,12,12]],"date-time":"2005-12-12T00:00:00Z","timestamp":1134345600000},"content-version":"vor","delay-in-days":3420,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1996,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Objective<\/jats:italic>. Autoantibodies directed against centromere proteins (CENPs) are a serologic feature in some patients with systemic sclerosis (SSc). Previous studies have focused on autoantibodies to CENPs A, B, and C. CENP\u2010E is a recently described 312\u2010kd protein that also localizes to the centromere. Therefore, we studied the presence of autoantibodies to recombinant CENP\u2010E in patients with SSc.<\/jats:p><jats:p><jats:italic>Methods<\/jats:italic>. Sixty sera from patients with the SSc spectrum of diseases were screened for the presence of autoantibodies against CENP\u2010E, by indirect immunofluorescence and immunoblotting using recombinant CENPE protein. HLA class II alleles were determined by DNA oligotyping.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>. Among the SSc sera, 15 of 60 (25%) demonstrated antibody reactivity with recombinant CENP\u2010E, and 14 of these 15 sera (93%) had antibodies directed against another CENP. Anti\u2013CENP\u2010E was seen in 13 of 30 sera with anti\u2010CENP (43%). All patients with anti\u2013CENP\u2010E had a limited form of SSc, known as the CREST variant (calcinosis, Raynaud's phenomenon, esophageal dysmotility, sclerodactyly, telangiectasias). When patients with anti\u2010CENPs A, B, or C were compared with patients with anti\u2013CENP\u2010E, no unique clinical features in the anti\u2013CENP\u2010E positive group were identified. Ninety\u2010three percent of the patients with anti\u2013CENP\u2010E had HLA\u2013DQB1 alleles that had polar amino acids at position 26 (primarily DQB1*05), similar to patients with other CENP autoantibodies.<\/jats:p><jats:p><jats:italic>Conclusion<\/jats:italic>. Antibodies to CENP\u2010E are common in patients with SSc, and are seen in higher frequency in sera from patients with a limited form, or CREST variant, of the disease.<\/jats:p>","DOI":"10.1002\/art.1780390813","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T12:02:15Z","timestamp":1171454535000},"page":"1355-1361","source":"Crossref","is-referenced-by-count":33,"title":["The centromere kinesin\u2010like protein, CENP\u2010E. 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