{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:20:55Z","timestamp":1760188855732},"reference-count":20,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2005,12,12]],"date-time":"2005-12-12T00:00:00Z","timestamp":1134345600000},"content-version":"vor","delay-in-days":3389,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis & Rheumatism"],"published-print":{"date-parts":[[1996,9]]},"abstract":"Abstract<\/jats:title>Objective<\/jats:italic>. To localize the epitope(s) bound by anti\u2013PM\u2010Scl antibodies in the N\u2010terminal half of the 100\u2010kd protein, the major antigen of the PM\u2010Scl complex.<\/jats:p>Methods<\/jats:italic>. Investigations were performed by immunoblotting 20 anti\u2013PM\u2010Scl positive sera against bacterially expressed, polymerase chain reaction\u2013derived deletion mutants of the S1 fragment (amino acids 11\u2013437), enzyme\u2010linked immunosorbent assay (ELISA) screening against synthesized serial octapeptides, and ELISA screening, with anti\u2013PM\u2010Scl positive sera, against a synthesized 21\u2013amino acid peptide covering the active region.<\/jats:p>Results<\/jats:italic>. Anti\u2013PM\u2010Scl positive sera retained full immunoblot activity with fragment 207\u2013436 and most activity with fragment 11\u2013241, but had markedly decreased activity against fragments 236\u2013436 and 11\u2013212, indicating a major epitope in the aa 207\u2013241 region. Fusion proteins with smaller fragments localized this activity between aa 226 and aa 246. Of 42 anti\u2013S1\u2010positive, anti\u2013PM\u2010Scl positive sera tested by ELISA against a synthetic peptide of this region, 36 were definitely positive, 4 borderline, and 2 negative. Similar activity was seen with a peptide from which proline 228 was deleted. Three additional epitope areas were found in S1, but each reacted with only a few sera. Anti\u2013PM\u2010Scl positive sera did not react with any octapeptide spanning the major epitope area (aa 207\u2013246).<\/jats:p>Conclusion<\/jats:italic>. The main immunoblot epitope of the PM\u2010Scl 100\u2010kd protein is within a central area of 21 aa (aa 226\u2013246), but is longer than the usual linear epitope. This peptide may be useful in patient testing. Three minor epitopes in S1 may also be recognized by some sera.<\/jats:p>","DOI":"10.1002\/art.1780390920","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T12:01:54Z","timestamp":1171454514000},"page":"1588-1595","source":"Crossref","is-referenced-by-count":14,"title":["Epitope analysis of the major reactive region of the 100\u2010kd protein of PM\u2010Scl autoantigen"],"prefix":"10.1002","volume":"39","author":[{"given":"Qun","family":"Ge","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yajuan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judith A.","family":"James","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ira N.","family":"Targoff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,12]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1016\/S0889-857X(21)00070-3","article-title":"Immune manifestations of inflammatory muscle disease","volume":"20","author":"Targoff IN","year":"1994","journal-title":"Rheum Dis Clin North Am"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00915286"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780351014"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-199211000-00001"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780330508"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780271011"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115895"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780280221"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"3802","DOI":"10.4049\/jimmunol.137.12.3802","article-title":"Immunolocalization and partial characterization of a nucleolar autoantigen (PM\u2010Scl) associated with polymyositis\/scleroderma overlap syndromes","volume":"137","author":"Reimer G","year":"1986","journal-title":"J Immunol"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1990.tb05291.x"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.173.4.941"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.176.4.973"},{"key":"e_1_2_1_14_2","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/S0889-857X(21)00738-9","article-title":"Autoantibodies in scleroderma","volume":"18","author":"Rothfield NF","year":"1992","journal-title":"Rheum Dis Clin North Am"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780371007"},{"key":"e_1_2_1_16_2","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.4049\/jimmunol.144.5.1737","article-title":"Autoantibodies to aminoacyl\u2010transfer RNA synthetases for isoleucine and glycine: two additional synthetases are antigenic in myositis","volume":"144","author":"Targoff IN","year":"1990","journal-title":"J Immunol"},{"issue":"9","key":"e_1_2_1_17_2","first-page":"S150","article-title":"Detection and quantification of human anti\u2010PM\u2010Scl autoantibodies using recombinant PM\u2010Scl protein (abstract)","volume":"36","author":"Ge Q","year":"1993","journal-title":"Arthritis Rheum"},{"key":"e_1_2_1_18_2","doi-asserted-by":"crossref","first-page":"2074","DOI":"10.4049\/jimmunol.148.7.2074","article-title":"Linear epitope mapping of an Sm B\/B polypeptide","volume":"148","author":"James JA","year":"1992","journal-title":"J Immunol"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00918804"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.181.2.453"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.19.7419"}],"container-title":["Arthritis & Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.1780390920","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.1780390920","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T01:19:11Z","timestamp":1700702351000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.1780390920"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,9]]},"references-count":20,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1996,9]]}},"alternative-id":["10.1002\/art.1780390920"],"URL":"https:\/\/doi.org\/10.1002\/art.1780390920","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,9]]}}}