{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T17:55:18Z","timestamp":1757699718750},"reference-count":45,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,12,12]],"date-time":"2005-12-12T00:00:00Z","timestamp":1134345600000},"content-version":"vor","delay-in-days":3177,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1997,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Objective.<\/jats:italic> Up to 42% of patients with Felty's syndrome (FS) have peripheral blood expansions of CD3+, CD8+ large granular lymphocytes (LGLs). The aim of this study was to determine whether the T cell receptor (TCR) \u03b1\u2010 and \u03b2\u2010chain sequences of these expansions from different patients have features in common that would support the hypothesis of an antigen\u2010driven process.<\/jats:p><jats:p><jats:italic>Methods.<\/jats:italic> Extraction of RNA from peripheral blood lymphocytes followed by synthesis of complementary DNA, inverse polymerase chain reaction (PCR) with TCR\u2010specific primers, bacteriophage transformation, and sequencing of PCR products.<\/jats:p><jats:p><jats:italic>Results.<\/jats:italic> Structural analysis of TCR \u03b2\u2010chain usage in such patients demonstrated a junctional region motif comprising the amino acids \u2010LG\u2010 or \u2010RG\u2010 in 7 of 14 clonal sequences and the motif \u2010GXG\u2010 in 8 of 14. A biased \u03b1\u2010chain junctional region usage of a hydrophobic and\/or basic amino acid at position 2 was seen in 5 of 8 expanded sequences. These features differed significantly from control sequences.<\/jats:p><jats:p><jats:italic>Conclusion.<\/jats:italic> Given current models of TCR\u2013peptide\u2013major histocompatibility complex interaction, these observations are consistent with an antigen\u2010driven, rather than a superantigen\u2010driven, process in at least a subgroup of patients with FS.<\/jats:p>","DOI":"10.1002\/art.1780400405","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T12:46:42Z","timestamp":1171457202000},"page":"615-623","source":"Crossref","is-referenced-by-count":16,"title":["T cell receptor \u03b1\u2010chain and \u03b2\u2010chain junctional region homology in clonal CD3+, CD8+ T lymphocyte expansions in Felty'S syndrome"],"prefix":"10.1002","volume":"40","author":[{"given":"Simon J.","family":"Bowman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret 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