{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:23:54Z","timestamp":1762100634467},"reference-count":41,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,12,12]],"date-time":"2005-12-12T00:00:00Z","timestamp":1134345600000},"content-version":"vor","delay-in-days":3147,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1997,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Objective.<\/jats:italic> To determine whether immunization of healthy non\u2010autoimmune mice with 52\u2010kd SS\u2010A\/Ro induces a secondary antibody response to other components of the 48\u2010kd SS\u2010B\/La\u201060\u2010kd SS\u2010A\/Ro RNP complex and vice versa, since anti\u201052\u2010kd antibodies have been invariably linked to these antigens in patients with Sj\u00f6gren's syndrome and in mothers whose children have neonatal lupus.<\/jats:p><jats:p><jats:italic>Methods.<\/jats:italic> Female BALB\/c mice were immunized with 100 \u03bcg of 6 \u00d7 His recombinant human 48\u2010kd SS\u2010B\/La, 52\u2010kd SS\u2010A\/Ro, or 60\u2010kd SS\u2010A\/Ro proteins, or the 6 \u00d7 His polypeptide control, each purified by Ni<jats:sup>2+<\/jats:sup> affinity chromatography. Mice subsequently received booster injections with 50 \u03bcg of the same antigen every 10\u201321 days. Immune responses were measured by enzyme\u2010linked immunosorbent assay (ELISA), immunoblotting of recombinant antigens, and immunoprecipitation of <jats:sup>35<\/jats:sup>S\u2010methionine\u2010labeled in vitro translation products.<\/jats:p><jats:p><jats:italic>Results.<\/jats:italic> Immunization with 48\u2010kd SS\u2010B\/La resulted in anti\u201048\u2010kd SS\u2010B\/La antibodies within 45 days, followed 10 days later by a secondary response to 52\u2010kd SS\u2010A\/Ro, as measured by ELISA. Antibody spreading to 60\u2010kd SS\u2010A\/Ro was not detected. Immunization with 52\u2010kd SS\u2010A\/Ro resulted in rapid high\u2010titer anti\u201052\u2010kd SS\u2010A\/Ro responses within 27 days. Spreading to 48\u2010kd SS\u2010B\/La occurred in only 1 mouse and 60\u2010kd SS\u2010A\/Ro was detected in a minority of the mice after prolonged antigen exposure. Immunization with 60\u2010kd SS\u2010A\/Ro led to anti\u201060\u2010kd SS\u2010A\/Ro responses within 37 days, followed 3 months later by low\u2010titer anti\u201048\u2010kd SS\u2010B\/La and anti\u201052\u2010kd SS\u2010A\/Ro antibodies. All primary immune responses were confirmed by immunoblotting and immunoprecipitation. While immunoblotting of the recombinant proteins revealed reciprocal intermolecular spreading in the majority of mice, immunoprecipitation clearly demonstrated that predominant spreading was generated after immunization with 48\u2010kd SS\u2010B\/La, which consistently resulted in antibodies to 52\u2010kd SS\u2010A\/Ro.<\/jats:p><jats:p><jats:italic>Conclusion.<\/jats:italic> The murine responses observed in the present study, demonstrating reciprocal intermolecular spreading to 48\u2010kd SS\u2010B\/La, 52\u2010kd SS\u2010A\/Ro, and 60\u2010kd SS\u2010A\/Ro, support the linkage of 52\u2010kd SS\u2010A\/Ro with the other proteins, despite their as\u2010yet\u2010undetected association in vivo. The marked recruitment of anti\u201052\u2010kd SS\u2010A\/Ro responses elicited by 48\u2010kd SS\u2010B\/La may provide a lead to exploring the physical interaction, direct or indirect, of 52\u2010kd SS\u2010A\/Ro with the SS\u2010A\/Ro\u2010SS\u2010B\/La RNP particle and its presentation to the immune system. These data should facilitate the establishment of a murine model of neonatal lupus.<\/jats:p>","DOI":"10.1002\/art.1780400523","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T12:45:20Z","timestamp":1171457120000},"page":"936-944","source":"Crossref","is-referenced-by-count":58,"title":["The 52\u2010kd protein as a target of intermolecular spreading of the immune response to components of the SS\u2010A\/Ro\u2010SS\u2010B\/La complex"],"prefix":"10.1002","volume":"40","author":[{"given":"Chung\u2010E","family":"Tseng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward K. 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