{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T11:36:32Z","timestamp":1771068992042,"version":"3.50.1"},"reference-count":62,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,12,12]],"date-time":"2005-12-12T00:00:00Z","timestamp":1134345600000},"content-version":"vor","delay-in-days":3147,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1997,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Objective.<\/jats:italic> The association of reactive arthritis (ReA) with HLA\u2010B27 and the presence of bacterial antigen in joints with ReA suggest that bacterial peptides might be presented by the HLA\u2010B27 molecule and thus stimulate CD8 T cells. This study was performed to investigate the B27\u2010restricted cytotoxic T lymphocyte (CTL) response to <jats:italic>Chlamydia trachomatis<\/jats:italic>, using the model of HLA\u2010B27 transgenic mice.<\/jats:p><jats:p><jats:italic>Methods.<\/jats:italic> CBA (H\u20102<jats:sup>k<\/jats:sup>) mice homozygous for HLA\u2010B*2705 and human \u03b2<jats:sub>2<\/jats:sub>\u2010microglobulin expression were immunized with <jats:italic>C trachomatis<\/jats:italic> or with the chlamydial 57\u2010kd heat\u2010shock protein (hsp57) coupled to latex beads. Cytotoxicity of lymphocytes from in vivo\u2014primed transgenic mice was tested against <jats:italic>C trachomatis<\/jats:italic>\u2014infected targets. Blocking experiments were performed with monoclonal antibodies (MAb) against class I major histocompatibility complex molecules.<\/jats:p><jats:p><jats:italic>Results.<\/jats:italic> A <jats:italic>Chlamydia<\/jats:italic>\u2010specific lysis of both B27\u2010transfected and nontransfected target cells was observed. This response could be inhibited by anti\u2010B27 and anti\u2010H2 MAb. CTL from mice immunized with hsp57 were not able to lyse <jats:italic>Chlamydia<\/jats:italic>\u2010infected target cells, and <jats:italic>Chlamydia<\/jats:italic>\u2010specific CTL could not destroy targets loaded with hsp57.<\/jats:p><jats:p><jats:italic>Conclusion.<\/jats:italic> These results suggest the existence of at least 2 CTL populations in this mouse model: one recognizing peptide of bacteria\u2010infected cells restricted by HLA\u2010B*2705 and the other recognizing peptide of bacteria\u2010infected cells restricted by the murine H\u20102K<jats:sup>k<\/jats:sup> molecule. It does not appear that hsp57 is a major target for the CD8 T cell response directed against <jats:italic>Chlamydia.<\/jats:italic> This animal model opens the way for identifying bacterial epitopes presented by HLA\u2010B27, and might thus help to clarify the pathogenesis of B27\u2010associated diseases.<\/jats:p>","DOI":"10.1002\/art.1780400524","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T12:45:20Z","timestamp":1171457120000},"page":"945-954","source":"Crossref","is-referenced-by-count":21,"title":["Recognition of chlamydial antigen by HLA\u2010B27\u2010restricted cytotoxic T cells in HLA\u2010B*2705 transgenic CBA (H\u20102<sup>k<\/sup>) 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