{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T07:30:14Z","timestamp":1780471814746,"version":"3.54.1"},"reference-count":21,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2005,12,12]],"date-time":"2005-12-12T00:00:00Z","timestamp":1134345600000},"content-version":"vor","delay-in-days":2994,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1997,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Objective<\/jats:italic>. To extend our work on the mapping of B cell epitopes on nucleosomal high mobility group (HMG) proteins in the sera of patients with juvenile rheumatoid arthritis (JRA).<\/jats:p><jats:p><jats:italic>Methods<\/jats:italic>. Seventy\u2010seven pauciarticular\u2010onset JRA serum samples from antinuclear antibody (ANA)\u2010 positive patients and 42 polyarticular\u2010onset JRA patient sera found to react with HMG\u20102 by immunoblotting were used in this study. To identify B cell epitopes on HMG\u20102, recombinant HMG\u20102 protein fragments were used in enzyme\u2010linked immunosorbent assay (ELISA) and in competition ELISA experiments with a set of overlapping synthetic peptides. Fine epitope mapping was achieved by oligopeptide synthesis, followed by immunoblotting.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>. Pauciarticular, but not polyarticular, JRA patient sera were found to recognize a lysine\u2010rich major epitope (KKGKKKDP), which is located in the linker region of the HMG box domains of the HMG\u20102 nonhistone chromosomal protein. No significant immunoreactions were observed in sera from ANA\u2010negative JRA patients and in sera from children with nonrheumatic diseases, indicating that this epitope seems to be specific for pauciarticular\u2010onset JRA.<\/jats:p><jats:p><jats:italic>Conclusion<\/jats:italic>. In addition to our previous finding that JRA sera will react with a defined epitope on HMG\u201017, pauciarticular JRA patient sera were also found to recognize a defined epitope on the HMG\u20102 protein, thus suggesting the importance of this epitope in the etiology of JRA.<\/jats:p>","DOI":"10.1002\/art.1780401012","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T12:15:21Z","timestamp":1171455321000},"page":"1803-1809","source":"Crossref","is-referenced-by-count":18,"title":["Antibodies against A peptide sequence located in the linker region of the HMG\u20101\/2 box domains in sera from patients with juvenile rheumatoid arthritis"],"prefix":"10.1002","volume":"40","author":[{"given":"Frank","family":"Jung","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunter","family":"Neuer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Friedlinde A.","family":"Bautz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,12,12]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780170613"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(78)81148-2"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780330910"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780350418"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.3109\/08916939409014655"},{"issue":"2","key":"e_1_2_1_7_2","first-page":"195","article-title":"Current proposed revision of JRA criteria","volume":"20","author":"Brewer EJ","year":"1977","journal-title":"Arthritis Rheum"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-372180-8.50014-7"},{"key":"e_1_2_1_9_2","first-page":"315","article-title":"Automated multiple peptide synthesis","volume":"5","author":"Gausepohl H","year":"1992","journal-title":"Pept Res"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4020(01)85612-X"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(89)70049-5"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(93)90022-F"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60641-0"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1993.tb05776.x"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.6.3824"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/344830a0"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/346240a0"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.177.5.1367"},{"key":"e_1_2_1_19_2","doi-asserted-by":"crossref","first-page":"3516","DOI":"10.4049\/jimmunol.154.7.3516","article-title":"Self peptides in the initiation of lupus autoimmunity","volume":"154","author":"Brockenstedt LK","year":"1995","journal-title":"J Immunol"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.3.875"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780380403"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0952-7915(95)80054-9"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.1780401012","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.1780401012","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T16:37:26Z","timestamp":1700757446000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.1780401012"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,10]]},"references-count":21,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1997,10]]}},"alternative-id":["10.1002\/art.1780401012"],"URL":"https:\/\/doi.org\/10.1002\/art.1780401012","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,10]]}}}