{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T06:37:41Z","timestamp":1770014261050,"version":"3.49.0"},"reference-count":18,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,2,5]],"date-time":"2004-02-05T00:00:00Z","timestamp":1075939200000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2004,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To compare the autoantigenicity of the recently described N\u2010terminally elongated PM\u2013Scl\u201075 protein with that of PM\u2013Scl\u2010100 and the originally defined PM\u2013Scl\u201075 polypeptide, and to determine its value for analyzing sera from patients with the polymyositis (PM)\/scleroderma overlap syndrome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Serum samples obtained from patients with the PM\/scleroderma overlap syndrome and from patients with several other diseases were analyzed for the presence of autoantibodies reactive with recombinant PM\u2013Scl\u2010100 and PM\u2013Scl\u201075 (both the original and the longer form) proteins, in an enzyme\u2010linked immunosorbent assay (ELISA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Autoantibodies recognizing the longer PM\u2013Scl\u201075 protein isoform were detected in 28% of the patients with PM\/scleroderma. This percentage is slightly higher than that for PM\u2013Scl\u2010100 (25%) and is significantly higher than that for the previously defined PM\u2013Scl\u201075 protein (11%). In addition, we identified a significant number of patients who had anti\u2013PM\u2013Scl\u201075 but not anti\u2013PM\u2013Scl\u2010100 antibodies. This finding contrasts with what has been previously reported for the shorter version of the PM\u2013Scl\u201075 protein.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our data indicate that use of the long PM\u2013Scl\u201075 isoform in addition to PM\u2013Scl\u2010100 in ELISAs significantly increases the number of patients in whom anti\u2013PM\u2010Scl autoantibodies can be detected.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.20056","type":"journal-article","created":{"date-parts":[[2004,2,9]],"date-time":"2004-02-09T18:29:18Z","timestamp":1076351358000},"page":"565-569","source":"Crossref","is-referenced-by-count":60,"title":["PM\u2013Scl\u201075 is the main autoantigen in patients with the polymyositis\/scleroderma overlap syndrome"],"prefix":"10.1002","volume":"50","author":[{"given":"Reinout","family":"Raijmakers","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manfred","family":"Renz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Wiemann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilma Vree","family":"Egberts","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans Peter","family":"Seelig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walther J.","family":"van Venrooij","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ger J. M.","family":"Pruijn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,5]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI108616"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1990.tb05291.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.173.4.941"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.176.4.973"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.16.3187"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(02)00947-6"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00578-5"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M007603200"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/ar389"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780351014"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.60.2.116"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-199211000-00001"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M302488200"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780230510"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197502132920706"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(98)00170-7"},{"key":"e_1_2_6_18_2","first-page":"5517","article-title":"CML28 is a broadly immunogenic antigen, which is overexpressed in tumor cells","volume":"62","author":"Yang XF","year":"2002","journal-title":"Cancer Res"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780371007"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.20056","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.20056","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T14:51:11Z","timestamp":1696949471000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.20056"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,2]]},"references-count":18,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2004,2]]}},"alternative-id":["10.1002\/art.20056"],"URL":"https:\/\/doi.org\/10.1002\/art.20056","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,2]]}}}