{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T18:31:16Z","timestamp":1776277876517,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2004,6,3]],"date-time":"2004-06-03T00:00:00Z","timestamp":1086220800000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2004,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate the regulation of acute\u2010phase serum amyloid A (A\u2010SAA) production in inflamed synovial tissue, and to elucidate a possible pathophysiologic role in the induction of matrix metalloproteinase (MMP) release by fibroblast\u2010like synoviocytes (FLS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Synovial tissue samples were obtained by arthroscopic biopsy from the knee joints of patients with inflammatory arthritis. Primary cultures of FLS from patients with rheumatoid arthritis (RA), psoriatic arthritis, sarcoid arthritis, and undifferentiated arthritis were established. Total RNA was extracted from FLS and analyzed by reverse transcription\u2013polymerase chain reaction (PCR) using specific primers for A\u2010SAA and formyl peptide receptor\u2013like 1 (FPRL1), an A\u2010SAA receptor. Southern blot analysis confirmed the PCR products generated. Immunohistochemical analysis demonstrated the expression of A\u2010SAA protein production by several synovial cell populations, and immunofluorescence analysis confirmed A\u2010SAA colocalization with the macrophage marker CD68. Primary FLS cultures stimulated with recombinant human A\u2010SAA resulted in dose\u2010dependent MMP\u20101 and MMP\u20103 production, as measured by an enzyme\u2010linked immunosorbent assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A\u2010SAA messenger RNA (mRNA) and FPRL1 mRNA were present in FLS, macrophages, and endothelial cells isolated from the synovial tissue of patients with RA and other categories of inflammatory arthritis. A\u2010SAA expression was regulated by proinflammatory cytokines and occurred in association with FPRL1 expression in FLS and endothelial cells, which is consistent with a biologic role at the sites of inflammation. Recombinant human A\u2010SAA induced both MMP\u20101 and MMP\u20103 secretion by FLS. The mean fold increases in A\u2010SAA\u2013induced MMP\u20101 and MMP\u20103 production were 2.6 and 10.6, respectively, compared with 7.6\u2010fold and 41.9\u2010fold increases in interleukin\u20101\u03b2\u2013induced MMP\u20101 and MMP\u20103 production.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The up\u2010regulation of the A\u2010SAA and FPRL1 genes in inflamed synovial tissue suggests an important role in the pathophysiology of inflammatory arthritis. A\u2010SAA induces the production of MMPs. Therapeutic targeting of A\u2010SAA, or FPRL1, may modulate pathophysiologic pathways that are associated with matrix degradation in patients with RA and other forms of progressive inflammatory arthritis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.20301","type":"journal-article","created":{"date-parts":[[2004,6,3]],"date-time":"2004-06-03T17:01:37Z","timestamp":1086282097000},"page":"1788-1799","source":"Crossref","is-referenced-by-count":119,"title":["Local expression of the serum amyloid A and formyl peptide receptor\u2013like 1 genes in synovial tissue is associated with matrix metalloproteinase production in patients with inflammatory arthritis"],"prefix":"10.1002","volume":"50","author":[{"given":"Rosemary","family":"O'Hara","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evelyn P.","family":"Murphy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander 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